Diagenode

Comparison of the S. cerevisae chromatin shearing with Covaris™ S2 and Bioruptor® Pico

The chromatin shearing is very important step for all kind of ChIP analysis followed by next-generation sequencing. The efficiency, reproducibility and straightforwardness are the most important characteristics for shearing. The using of S. cerevisiae as a model organism is even more complicated because they have a cell wall which require special lysis and can effect shearing. In this protocol we compared the yeast chromatin shearing by Covaris™ S2 and Bioruptor® Pico, and the chromatin crosslinking by 16% methanol-free and 37% methanol-containing formaldehyde.

関連商品

  • Bioruptor-pico-next-gen-sequencing
    B01060010
    Picoruptor Sonicator

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  • EpiChrom
    Umea Sweden
    Feb 27-Feb 28, 2020
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