Diagenode

Comparison of the S. cerevisae chromatin shearing with Covaris™ S2 and Bioruptor® Pico

The chromatin shearing is very important step for all kind of ChIP analysis followed by next-generation sequencing. The efficiency, reproducibility and straightforwardness are the most important characteristics for shearing. The using of S. cerevisiae as a model organism is even more complicated because they have a cell wall which require special lysis and can effect shearing. In this protocol we compared the yeast chromatin shearing by Covaris™ S2 and Bioruptor® Pico, and the chromatin crosslinking by 16% methanol-free and 37% methanol-containing formaldehyde.

相关产品

  • Bioruptor pico next gen sequencing
    B01060010
    Bioruptor® Pico sonication device

活动

  • CNET 2022
    Gif-Sur-Yvette, France
    Jul 4-Jul 6, 2022
 查看所有活动

新闻

 查看所有新闻


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics