Diagenode

Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor®

Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene, or a mineral oil-based method which can be time consuming and messy. Claire Josse and colleagues from the Human Genetic Laboratory at the GIGA - University of Liège have developed a new protocol combining the Bioruptor® Pico with the AllPrep FFPE DNA/RNA kit from Qiagen® to ef ciently prepare FFPE samples for Next-Generation Sequencing. The Bioruptor® Pico removes the parafin and rehydrates the tissue in just one solvent-free step followed by a mild crosslink reversal to preserve DNA and RNA integrity. The nucleic acids are then extracted with the AllPrep FFPE DNA/RNA kit and sheared using the Bioruptor® Pico to the desired length for sequencing library preparation.

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イベント

  • Japanese Biochemical Society
    Yokohama
    Sep 18-Sep 20, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    Atlanta, Georgia
    Sep 19, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    RTP, North Carolina
    Sep 20, 2019
  • EpiGeneSys
    London
    Sep 22-Sep 24, 2019
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