Diagenode

Epigenetics DNA Methylation

Methylated DNA immunoprecipitation

The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.

How it works

In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.

Overview

Made for Methylated DNA immunoprecipitation

Cat. No.ProductFormatPrice
C02010031 hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $495.00
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental cond...
1 unit
C30010014 2 ml microtubes for SX-8G IP-Star® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System.
100 pc $35.00
C15410204-100 5-Carboxylcytosine (5-caC) polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against 5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to BSA.  
100 µg $295.00
C15410205-50 5-hydroxymethylcytosine (5-hmC) polyclonal antibody (rabbit) - Classic
Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.
50 μg $295.00
C15200081-100 5-methylcytosine (5-mC) monoclonal antibody 33D3 - Premium
The 5-methylcytosine antibody (clone 33D3) is the most published and widely used antibody for DNA methylation analysis. It has been validated for Methylated ...
100 µg/100 µl
(100 MeDIP reactions)
$460.00
C15200006-100 5-methylcytosine (5-mC) monoclonal antibody cl. b - Classic
Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to ovalbumine.
100 µg/50 µl $360.00
C02010034 Auto hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $495.00
C02010012 Auto MeDIP kit
Perform Automated MeDIP (Methylated DNA Immunoprecipitation[application MeDIP] to estimate DNA methylation status of your sample using the 5-methylcytidine a...
100 rxns $1,500.00
C02040012 DNA Methylation control package
The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your...
40 rxns $245.00
C02010030 hMeDIP kit x16 (monoclonal rat antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $495.00
C02010032 hMeDIP kit x16 (polyclonal rabbit antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $495.00
C11000002 Hydroxymethyl dCTP
Hydroxymethyl dCTP can be used as a substrate for MethylTaq DNA polymerase (Cat. No. AF-103-0250) permitting the amplification of DNA containi...
250 µl $495.00
C02010021 MagMeDIP kit
Perform MeDIP (Methylated DNA Immunoprecipitation) to estimate DNA methylation status of your sample using the 5-methylcytidine antibody. The development of ...
48 rxns $595.00
C15400001 Mouse IgG
The negative Ctrl IgG from mouse has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses ...
15 µg/15 µl $35.00
C15410206 Rabbit IgG
The negative Ctrl IgG from rabbit has been extensively validated in chromatin immunoprecipitation assays (ChIP). It contains a spectrum of the IgG subclasses...
250 µg/250 µl $55.00
C15420001 Rat IgG
The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in ser...
25 µg/ 25 µl $60.00

Related protocols

MeDIP-sequencing protocol with the MagMedip kit
MeDIP-sequencing protocol with the MagMedip kit.
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    Crete, Greece
    Oct 3-Oct 8, 2016
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    Oct 3-Oct 4, 2016
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    Puerto Varas, Chile
    Sep 27-Sep 30, 2016
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