Diagenode

Epigenetics DNA Methylation

Complete solutions for DNA methylation studies

Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.

DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. In vivo, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.



  • Learn more
    5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.


Main DNA methylation technologies

Overview of the three main approaches for studying DNA methylation.

  1. Chemical modification with bisulfite – Bisulfite conversion
  2. Enrichment of methylated DNA (including MeDIP and MBD)
  3. Treatment with methylation-sensitive or dependent restriction enzymes

 

Description Features
Bisulfite conversion Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.
  • Single nucleotide resolution
  • Quantitative analysis - methylation rate (%)
  • Gold standard and well studied
  • Compatible with automation
Methylated DNA enrichment (Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.
  • Resolution depends on the fragment size of the enriched methylated DNA (300 bp)
  • Qualitative analysis
  • Compatible with automation
Restriction enzyme-based digestion Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.
  • Determination of methylation status is limited by the enzyme recognition site
  • Easy to use

  • Bisulfite conversion

    Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from...
  • Methylated DNA immunoprecipitation

    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is performed as follows: Genomic ...
  • Methylbinding domain protein

    The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. The ...

Made for Epigenetics DNA Methylation

Cat. No.ProductFormatPrice
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental cond...
1 unit
C15200003 5-methylcytosine (5-mC) monoclonal antibody for ICC/IF - Classic
Monoclonal antibody raised in mouse against 5-mC (5-methylcytosine) conjugated to BSA. 
50 μg/21 μl $295.00
C02040011 5-hmC, 5-mC & cytosine DNA standard pack for hMeDIP
The hydroxymethylated DNA standard is a linear dsDNA that has been produced by PCR using the Diagenode’s MethylTaq DNA polymerase and Diageno...
40 rxns $175.00
C15200200-50 5-hydroxymethylcytosine (5-hmC) monoclonal antibody (mouse) - Classic
One of the only two monoclonal antibodies raised against 5-hydroxymethylcytosine (5-hmC). 5-hmC is a recently discovered DNA modification which res...
50 µg/50 µl $295.00
C15220001-50 5-hydroxymethylcytosine (5-hmC) monoclonal antibody (rat) - Classic
5-hmC is a DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Pre...
50 µg/32 µl $295.00
C15310210-100 5-hydroxymethylcytosine (5-hmC) polyclonal antibody (rabbit) - Classic
Polyclonal antibody raised against 5-hydroxymethylcytosine (5-hmC). 5-hmC is a recently discovered DNA modification which results from the enzymatic conversi...
100 µl $295.00
C02010034 Auto hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $495.00
C02010033 Auto hMeDIP kit x16 (monoclonal rat antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $495.00
C02010035 Auto hMeDIP kit x16 (polyclonal rabbit antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $495.00
C15410291 H3K18me2 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against Histone H3 (dimethyl Lys18), using a KLH-conjugated synthetic peptide.
50 μg $350.00
C15410292 H3K18me3 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against Histone H3 (trimethyl Lys18), using a KLH-conjugated synthetic peptide.
50 μg $350.00
C15410293 H3K27me3S28p polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against Histone H3 (trimethyl Lys27, p Ser28), using a KLH-conjugated synthetic peptide.
50 μg $350.00
C15410294 H3R2me2K4me2 polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against Histone H3 (sym-dimethyl Arg2, dimethyl Lys4), using a KLH-conjugated synthetic peptide.
50 μg $350.00
C15410300 H4K16ac polyclonal antibody - Classic
Polyclonal antibody raised in rabbit against Histone H4 (ac Lys16), using a KLH-conjugated synthetic peptide.
50 μg $350.00
C03020007 Pre-blocked Protein A/G beads for meDNA IP
This reagent is intended for MeDIP (Methylated DNA Immunoprecipitation).
300 µl $195.00
C06010010 PvuRts1l restriction enzyme
PvuRts1I is a restriction enzyme that selectively cleaves 5-hmC-containing DNA sequences. PvuRts1I is able not only to discriminate between 5-hmC and 5-mC bu...
100 µl $115.00
C15200206 RbAp48 monoclonal antibody - Classic
Alterantive names: RBBP4, CAF-1 subunit C, CAF-I P48, NURF55, Lin-53. Monoclonal antibody raised in mouse against retinoblastoma binding protein 4, using a ...
100 µg $350.00

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