Diagenode

Bioruptor® Plus sonication device

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Personalised trade in offer for your Bioruptor model

Temperature-controlled
Easy to use
Processing of 3-12 samples
Sample size 100 µl – 20 ml
Advanced timing control
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Catalog Number
Format
B01020001
(UCD-300 TM)
1 unit

The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).

TESTIMONIAL

To expedite the larger-scale phenotyping of pollen number difference within Arabidopsis family, we have modified an existing method more suitable for our purpose of pollen counting using a Bioruptor®. We grew four plants per genotype. Three flower buds per plant were harvested and dried at 65°C overnight. We collected flower buds with mature pollen, but before the anthers were opened (flower stage 13). We did not use the first and second flowers of the inflorescence, as they tend to show developmentally immature flower morphologies. 30 μl of 5% Tween-20 is added to the dried flower and samples are sonicated using the Bioruptor® Plus with high power mode for 10 cycles (sonication cycle: 30 sec ON, 30 sec OFF) to separate pollen grains from the anthers. Then, all pollen solutions are measured with a cell counter.

Misako Yamazaki, Evolutionary and Ecological Genomics (Kentaro Shimizu group), Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Switzerland
  • Which Bioruptor® Plus configuration is right for you?

    B01020001 - Bioruptor Plus® device with 1.5 ml (6 samples) tube holder & temperature controlled system (water cooler + single cycle valve) for recommended sample volume 100 µl - 300 µl

    B01020002 - Bioruptor Plus® device with 1.5 ml (6 samples) and 15 ml tube holders & temperature controlled system (water cooler + single cycle valve) for recommended sample volume 100 µl - 2 ml

    B01020003 - Bioruptor Plus® device with 0.5 ml (6 samples) tube holder & temperature controlled system (water cooler + single cycle valve) for recommended sample volume 50 µl - 100 µl 

  • Testimonials

    We use the Bioruptor sonication device and the antibody against the histone modification H3K4me3 in our lab. The Bioruptor device is used for the shearing of chromatin in ChIP-Seq experiments and for generation of protein lysates (whole cell extracts). Thanks to the Bioruptor device we achieve excellent and reproducible results in both applications. The H3K4me3 antibody is used in our ChIP-Seq experiments as well as Western Blotting. With this antibody we are able to generate highly enriched ChIP-Seq samples with extremely low background. In Western Blot we can detect one specific, strong band with the H3K4me3 antibody.

    Diagenode provides not only very good products for research but also an excellent customer support.

    Dr Lora Dimitrova, Charité-Universitätsmedizin Berlin, Germany
  • Protocols
    Activity Test - Procedure for immunoprecipitation of native chromatin proteins
    Proteins play role in almost all cellular processes, and the study of their activity is crucial f...
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    Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL
    For cell lysis, we highly recommend using 1.5 ml TPX microtubes (Cat. No. M-50050) or 10 ml tubes...
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    ChIP - Just 4 steps to foolproofing fragmentation for ChIP using Bioruptor® Standard, Plus or Pico
    Just follow these four steps for shearing chromatin, and you’ll be well on your way to exce...
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    ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus
    A review of the critical points for chromatin shearing.
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    Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico
    This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chro...
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    Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus
    Standard procedure to shear Chromatin from Yeast. 
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    DNA Shearing for Bioruptor® Standard and Plus
    For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-p...
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    FFPE DNA extraction protocol using the Bioruptor®
    The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, provid...
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    Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis
    Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck ...
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    Mass-Spectrometry: procedure for shotgun proteomics
    Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Sh...
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    Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus
    Protein extraction from tissues and cultured cells is the first step for many biochemical and ana...
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    RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit
    Isolation of intact RNA is essential for many techniques used in gene expression analysis. Effici...
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    TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
    The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein com...
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    Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®
    The regular protocol for the extraction of histone requires an acid extraction, making impossible...
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  • Applications
    Chromatin shearing
    The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, Diagenode's Bio... Read more
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
    RNA extraction
    Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption ... Read more
    Protein extraction
    Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such... Read more
    FFPE DNA extraction
    Diagenode's high yields FFPE DNA extraction using Bioruptor® is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraf... Read more
  • Documents
    Bioruptor® Sonicator Brochure BROCHURE
    Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp...
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    Bioruptor® Plus manual MANUAL
    Diagenode’s Bioruptor® Plus uses a gentle method of sonication to retain the integrity ...
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    Optimizing DNA Shearing for Next Generation Sequencing Library Preparation with the Bioruptor® POSTER
    The rapid adoption of next-generation, direct, and single- molecule sequencing technologies has m...
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    Which tubes for which Bioruptor®? QUICK GUIDE
    Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu...
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    Bacterial Cell Disruption using Bioruptor® Plus APPLICATION NOTE
    Disruption of bacterial cells is required for releasing biological molecules from within ...
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    Bioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing CASE STUDY
    Wouter Coppieters, PhD, and his colleagues operate the genomics core facility at the GIGA center,...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Bioruptor® Plus sonication device (Diagenode Cat# B01020001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4(+) memory T cells
    Kinkley S et al.
    The combinatorial action of co-localizing chromatin modifications and regulators determines chromatin structure and function. However, identifying co-localizing chromatin features in a high-throughput manner remains a technical challenge. Here we describe a novel reChIP-seq approach and tailored bioinformatic analys...

    UMI-4C for quantitative and targeted chromosomal contact profiling
    Omer Schwartzman, Zohar Mukamel, Noa Oded-Elkayam, Pedro Olivares-Chauvet, Yaniv Lubling, Gilad Landan, Shai Izraeli and Amos Tanay
    We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (umis) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can ...

    Histamine 1 receptor-Gβγ-cAMP/PKA-CFTR pathway mediates the histamine-induced resetting of the suprachiasmatic circadian clock
    Yoon Sik Kim, Young-Beom Kim, Woong Bin Kim, Seung Won Lee, Seog Bae Oh, Hee-Chul Han, C. Justin LeeEmail author, Christopher S. Colwell and Yang In Kim
    Background Recent evidence indicates that histamine, acting on histamine 1 receptor (H1R), resets the circadian clock in the mouse suprachiasmatic nucleus (SCN) by increasing intracellular Ca2+ concentration ([Ca2+]i) through the activation of CaV1.3 L-type Ca2+ channels and Ca2+-induced Ca2+ release from ryan...

    Standardizing chromatin research: a simple and universal method for ChIP-seq
    Laura Arrigoni, Andreas S. Richter, Emily Betancourt, Kerstin Bruder, Sarah Diehl, Thomas Manke and Ulrike Bönisch
    Here we demonstrate that harmonization of ChIP-seq workflows across cell types and conditions is possible when obtaining chromatin from properly isolated nuclei. We established an ultrasound-based nuclei extraction method (Nuclei Extraction by Sonication) that is highly effective across various organisms, cell ...

    HNF1β drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC)
    Filipa Lopes-Coelho, Sofia Gouveia-Fernandes, Luís G Gonçalves, Carolina Nunes, Inês Faustino, Fernanda Silva, Ana Félix, Sofia A Pereira, Jacinta Serpa
    Chemoresistance to platinum-based antineoplastic agents is a consistent feature among ovarian carcinomas; however, whereas high-grade serous carcinoma (OSC) acquires resistance during chemotherapy, ovarian clear cell carcinoma (OCCC) is intrinsically resistant. The main objective of this study was to explore, in vit...

    LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2
    Maryem A. Hussein, Elina Shrestha, Mireille Ouimet, Tessa J. Barrett, Sarah Leone, Kathryn J. Moore, Yann Hérault, Edward A. Fisher, Michael J. Garabedian
    High cholesterol and diabetes are major risk factors for atherosclerosis. Regression of atherosclerosis is mediated in part by the Liver X Receptor (LXR) through the induction of genes involved in cholesterol transport and efflux. In the context of diabetes, regression of atherosclerosis is impaired. We proposed tha...

    Sensitive detection of chromatin-altering polymorphisms reveals autoimmune disease mechanisms.
    Del Rosario RC, Poschmann J, Rouam SL, Png E, Khor CC, Hibberd ML, Prabhakar S
    Most disease associations detected by genome-wide association studies (GWAS) lie outside coding genes, but very few have been mapped to causal regulatory variants. Here, we present a method for detecting regulatory quantitative trait loci (QTLs) that does not require genotyping or whole-genome sequencing. The method...

    Population differentiation determined from putative neutral and divergent adaptive genetic markers in Eulachon (Thaleichthys pacificus, Osmeridae), an anadromous Pacific smelt.
    Candy JR, Campbell NR, Grinnell MH, Beacham TD, Larson WA, Narum SR
    Twelve eulachon (Thaleichthys pacificus, Osmeridae) populations ranging from Cook Inlet, Alaska and along the west coast of North America to the Columbia River were examined by restriction-site-associated DNA (RAD) sequencing to elucidate patterns of neutral and adaptive variation in this high geneflow species. A to...

    Silica nanoparticles induce oxidative stress, inflammation, and endothelial dysfunction in vitro via activation of the MAPK/Nrf2 pathway and nuclear factor-κB signaling.
    Caixia Guo,1,2 Yinye Xia,1,2 Piye Niu,1,2 Lizhen Jiang,1,2 Junchao Duan,1,2 Yang Yu,1,2 Xianqing Zhou,1,2 Yanbo Li,1,2 Zhiwei Sun1,2
    Abstract: Despite the widespread application of silica nanoparticles (SiNPs) in industrial, commercial, and biomedical fields, their response to human cells has not been fully elucidated. Overall, little is known about the toxicological effects of SiNPs on the cardiovascular system. In this study, SiNPs with a ...

    Step-by-step preparation of proteins for mass spectrometric analysis.
    Franz T, Li X.
    Nowadays, identification of proteins from biological samples by mass spectrometry is widely used. In principle there are two scenarios. Proteins are pre-fractionated in some way, e.g. by gel electrophoresis or are analyzed as complex mixture (shot gun). Shot gun proteomics became recently more popular, because of te...

    Epigenetic-Mediated Downregulation of μ-Protocadherin in Colorectal Tumours
    Bujko M, Kober P, Statkiewicz M, Mikula M, Ligaj M, Zwierzchowski L, Ostrowski J, Siedlecki JA
    Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorectal cancer (CRC). This study has compared MUPCDH (CDHR5) expression in ...

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