Diagenode

Universal Plant ChIP-seq kit

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Catalog Number
Format
Price
C01010152
24 rxns
$495.00

The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, maize, rice, tomato and poplar. This complete kit has been specifically optimized for plant chromatin extraction and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.

  • Characteristics
    • Universal compatiblity with a wide variety of plant species
    • Optimized and complete kit for start-to-finish plant ChIP
    • Includes plant-specific control primers and control antibody

    Successful ChIP-seq experiments for a variety of plants

    Arabidopsis

    Figure 1. ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.

    Poplar

    Figure 3. ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the MicroPlex Library Preparation™ kit from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.

    Tomato

    Figure 2. ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.

    Maize

    Figure 4. ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.

     

    Plant Species

    Validated antibodies

    Validated primer pairs

    Arabidopsis (Arabidopsis thaliana) H3K4me3 polyclonal antibody - Premium Arabidopsis Actin ATG primer pair
    H3K4me3 monoclonal antibody - Classic Arabidopsis FLC-ATG primer pair
    H3K9me2 polyclonal antibody - Classic Arabidopsis FLC-intron1 primer pair
    H3K9me3 polyclonal antibody - Classic
    H3K9/14ac polyclonal antibody - Classic
    H3K27me3 polyclonal antibody - Premium
    H3K27ac polyclonal antibody - Premium
    H3K36me3 polyclonal antibody - Premium
    Maize (Zea mays) H3K4me3 polyclonal antibody - Premium Maize B73 inner stem ZmB1-UTR primer pair
    H3K27me3 polyclonal antibody - Premium Maize B73 inner stem ZmCopia primer pair
    H3pan monoclonal antibody - Classic
    Tomato (Solanum lycopersicum) H3K4me3 polyclonal antibody - Premium Tomato leaves SlChr2-reg8 primer pair
    H3K9me2 polyclonal antibody - Classic Tomato leaves SlChr4-NC1 primer pair
    H3K27me3 polyclonal antibody - Premium
    H3pan monoclonal antibody - Classic
    Rice (Oriza sativa) H3K4me3 polyclonal antibody - Premium Rice seedlings OsChr4-reg9 primer pair
    H3K9me2 polyclonal antibody - Classic Rice seedlings OsMADS6 primer pair
    H3K36me3 polyclonal antibody - Premium
    Poplar (Populus trichocarpa, Populus tremula x alba) H3K4me3 polyclonal antibody - Premium Poplar xylem PtrCopia-orth primer pair
    H3K9ac polyclonal antibody - Classic Poplar xylem PtrMYBTF1 primer pair
    H3pan monoclonal antibody - Classic
    H3K27me3 polyclonal antibody - Premium

  • Applications
    ChIP-seq
    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomic researches, namely to investigate protein-DNA interaction on a ... Read more
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
  • Documents
    Universal Plant ChIP-seq kit MANUAL
    Manual of the Universal Plant ChIP-seq Kit
    Download
    Cell number estimation plants XLS FILE
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Universal Plant ChIP-seq kit (Diagenode Cat# C01010152). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Transcription Factor Interplay between LEAFY and APETALA1/CAULIFLOWER during Floral Initiation
    Goslin K. et al.
    The transcription factors LEAFY (LFY) and APETALA1 (AP1), together with the AP1 paralog CAULIFLOWER (CAL), control the onset of flower development in a partially redundant manner. This redundancy is thought to be mediated, at least in part, through the regulation of a shared set of target genes. However, whether the...

    Nitric oxide modulates histone acetylation at stress genes by inhibition of histone deacetylases
    Mengel A. et al.
    Histone acetylation, which is an important mechanism to regulate gene expression, is controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs). In animals, several HDACs are subjected to regulation by nitric oxide (NO), in plants however, it is unknown whether NO affects...

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