Diagenode

Optimizing DNA Shearing for Next Generation Sequencing Library Preparation with the Bioruptor®

The rapid adoption of next-generation, direct, and single- molecule sequencing technologies has made DNA shearing a critically important step in library template preparation. DNA sonication is the preferred method to prepare high quality, random, and size-appropriate sequencing libraries, and also ideal to fractionate chromatin for epigenetics studies. Here we present optimized sonication methods and protocols using the Bioruptor® sonicator to make genomic DNA libraries for the Illumina®, HeliScopeTM, SOLIDT and 454 platforms.

Related products

  • Bioruptor Plus Sonication Device
    B01020001
    Bioruptor® Plus sonication device

Events

  • Virtual ChIP workshop - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Virtual DNA Methylation - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Lausanne Genomics Days 2022
    Lausanne, Switzerland
    Feb 14-Feb 15, 2022
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News

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The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


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