Optimizing DNA Shearing for Next Generation Sequencing Library Preparation with the Bioruptor®
The rapid adoption of next-generation, direct, and single- molecule sequencing technologies has made DNA shearing a critically important step in library template preparation. DNA sonication is the preferred method to prepare high quality, random, and size-appropriate sequencing libraries, and also ideal to fractionate chromatin for epigenetics studies. Here we present optimized sonication methods and protocols using the Bioruptor®sonicator to make genomic DNA libraries for the Illumina®, HeliScopeTM, SOLIDT™and 454 platforms.
Get a quote
You are about to request a quote for Bioruptor® Plus sonication device. Fill out the form below and we will be in touch with you very soon.