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Bioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing

Wouter Coppieters, PhD, and his colleagues operate the genomics core facility at the GIGA center, University of Liège (Belgium). The center routinely performs a variety of Next-Generation Sequencing (NGS) applications including de novo sequencing, whole genome and amplicon sequencing, and targeted resequencing on an Illumina GAIIx analyzer. Coppieters recognizes that optimal sequencing results rely heavily on the quality and preparation of DNA libraries. Library preparation can be complex with multiple steps that require optimization. As Coppieters notes, “A prerequisite to obtaining high quality Next-Generation Sequencing data is to have libraries that have the desired insert size (November 2011)”.

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