Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico
This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.
Related product
C01010051
iDeal ChIP-seq kit for Histones
B01020001
Get a quote
You are about to request a quote for %s. Bioruptor® Plus sonication deviceFill out the form below and we will be in touch with you very soon.
To ensure you see the information most relevant to you, please select your country.
Please note that your browser will need to be configured to accept cookies.
