Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor^® Standard, Plus or Pico

This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.

Related product

C01010051

iDeal ChIP-seq kit for Histones
B01020014

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Bioruptor® Plus sonication device
C01010120

Elution Module
C30010013

0.5 ml Bioruptor® Plus Microtubes
C30010010-300

1.5 ml Bioruptor® Plus TPX microtubes

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Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico

Related product

iDeal ChIP-seq kit for Histones

Bioruptor® Plus sonication device

Elution Module

0.5 ml Bioruptor® Plus Microtubes

1.5 ml Bioruptor® Plus TPX microtubes

Events

Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor^® Standard, Plus or Pico