The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples.

Paul Datlinger and Christoph Bock, CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria
about Premium RRBS kit, Premium RRBS kit, RRBS Service (Reduced Representation Bisulfite Sequencing).

I have been doing ChIPs for a very long time and have tried many kits from different sources like Active Motif, Millipore/Upstate, and homemade reagents. The reproducibility and binding efficacy were never optimal for these until a colleague recommended the iDeal ChIP-seq Kit for Transcription Factors from Diagenode. I have done more than one hundred samples of ChIPs and ChIP-seq using this kit. The results are very consistent and the binding efficacy is higher than with all the other methods. I would definitely recommend this ChIP kit from Diagenode to anyone who is trying to do ChIP or ChIP-seq.

Researcher at Johns Hopkins University, School of Medicine
about iDeal ChIP-seq kit for Transcription Factors.

Our laboratory has used RRBS sevice of Diagenode on murine and human samples. The service was impeccable in each phase, from the sample preparation to bionformatic analysis because it was always customer-oriented. I highly recommend my colleagues to use the RRBS service from Diagenode.

Prof. Lucia Altucci, MD, PhD, Seconda Università degli Studi di Napoli, Dipartimento di Biochimica, Biofisica e Patologia generale .
about Premium RRBS kit, Premium RRBS kit, RRBS Service (Reduced Representation Bisulfite Sequencing).

The system performance was very good. High molecular weight DNA of different plants (Arabidopsis Alm, Hops polaris and wine Villard Blanc) was isolated and diluted to 50ng/µl or 70ng/µl. We aimed for fragment length 30kb at the volume 150µl.
We achieved good results and we will continue with bead purification and library preps for PacBio sequencing.  I like the user friendly interface of the Megaruptor® 2. Just two parameters had to be specified: volume of each sample and desired mean fragment size. The processed DNA has a narrow size distribution at around 30kb.

Christa Lanz and Julia Hildebrandt, Max Planck Institute for Developmental Biology, Tübingen, Germany
about Megaruptor® 2.

At the VIB Department of Molecular Genetics we strive to implement the best technologies in our workflows. Recent advances in long read sequencing using Oxford Nanopore technology have made the investigation of complex genomic variation in the human genome possible. A crucial step in the beginning of the library preparation is the shearing of genomic DNA, and this is where we implement the Megaruptor. The straightforward protocol is efficient, requires minimal sample handling and results in very reproducible fragment sizes. Another advantage is the consumable cost, which is lower than alternative technologies. For our applications we prefer shearing to lengths of 10 to 40kb, and for this the Megaruptor is the optimal solution.

Wouter de Coster and the Genomic Service Facility, VIB Department of Molecular Genetics, Antwerp, Belgium
about Megaruptor®.

High throughput sequencing has dramatically reduced sequencing costs and the development of duel indexing strategies makes it possible to combine 384 samples in a single sequencing run. By developing library preparation methods to enrich for retroviral integration sites and retroviral transcripts we have been able to produce large numbers of low cost libraries to track the evolution of infection and to characterise patterns of expression from our virus of interest. The Bioruptor® Pico has been essential part of our protocols and provides straightforward and consistent shearing of our samples.

Dr Keith Durkin, Unit of Animal Genomics, GIGA-R, Université de Liège , 4000 Liège, Belgium
about Bioruptor® Pico sonication device.

The Diagenode TDG recombinant protein (Cat. No. C23020101) is a very good tool. I used it as positive control in western blot analysis and during TDG ELISA activity assays. The protein is really active! Indeed the TDG recombinant protein perfectly works in glycosylase activity assays using marked oligos.

Dr Francesco Spallotta, Division of Cardiovascular Epigenetics, Goethe University, Frankfurt am Main, Germany

Diagenode Ago monoclonal antibody (Cat No. C15200167) immunoprecipitates the predicted miRNA target alleles very efficiently

Haruko Takeda, University of Liège, Belgium
about Ago (Argonautes) Antibody , Ago (Argonautes) Antibody - Classic, Ago (Argonautes) Antibody (sample size).

In life sciences, epigenetics is nowadays the most rapid developing field with new astonishing discoveries made every day. To keep pace with this field, we are in need of reliable tools to foster our research - tools Diagenode provides us with. From antibodies to automated solutions - all from one source and with robust support. Antibodies used in our lab: H3K27me3 polyclonal antibody – Premium, H3K4me3 polyclonal antibody – Premium, H3K9me3 polyclonal antibody – Premium, H3K4me1 polyclonal antibody – Premium, CTCF polyclonal antibody – Classic, Rabbit IgG.

Dr. Florian Uhle, Dept. of Anesthesiology, Heidelberg University Hospital, Germany
about H3K27me3 Antibody, H3K27me3 antibody (sample size), H3K4me3 Antibody, H3K4me3 Antibody (sample size), H3K9me3 Antibody, H3K4me1 Antibody (sample size), H3K4me1 Antibody, CTCF Antibody , CTCF Antibody (sample size), Rabbit IgG, IP-Star® Compact Automated System, H3K9me3 Antibody (sample size).

Using the Premium RRBS kit we quickly established the RRBS library preparation in our group. Diagenode's support on wet-lab matters as well as on bioinformatics was exceptionally customer-oriented and close, accelerating the establishment of the method.

Dr. Eduard Resch, Fraunhofer Insitute for Molecular Biology and Applied Ecology IME, Project Group Translational Medicine & Pharmacology TMP, Frankfurt am Main
about Premium RRBS kit, Premium RRBS kit.

We use the Bioruptor sonication device and the antibody against the histone modification H3K4me3 in our lab. The Bioruptor device is used for the shearing of chromatin in ChIP-Seq experiments and for generation of protein lysates (whole cell extracts). Thanks to the Bioruptor device we achieve excellent and reproducible results in both applications. The H3K4me3 antibody is used in our ChIP-Seq experiments as well as Western Blotting. With this antibody we are able to generate highly enriched ChIP-Seq samples with extremely low background. In Western Blot we can detect one specific, strong band with the H3K4me3 antibody.

Diagenode provides not only very good products for research but also an excellent customer support.

Dr Lora Dimitrova, Charité-Universitätsmedizin Berlin, Germany
about Bioruptor® Plus sonication device, H3K4me3 Antibody, H3K4me3 Antibody (sample size).

I have been a Diagenode customer for over two years now. I have used the antibodies against the histone modifications H3K4me3, H3K4me2, H3K4me1, H3k27me3, H3K9ac, H3K27ac etc. provided by Diagenode to perform western blots.The high level of specificity of these antibodies in Pristionchus pacificus samples, confirmed by using several negative and positive controls run in parallel with synchronized culture samples, ensured successful and reproducible results.

Also, I am satisfied with the service and the support of personnel of Diagenode. They are prompt in replying to my emails and very helpful with their advice. Thanks a lot!

Vahan Serobyan, Prof. Dr Ralf J. Sommer's Lab, Max Planck Institute for Developmental Biology, Tübingen, Germany

I work with Diagenode’s Plant ChIP-seq kit and shear the DNA on the Bioruptor Pico for the last year and I have to say that these two products saved my PhD project! Some time ago, our well-established ChIP protocol suddenly stopped to work and after long time of figuring out the reason, we invested into Bioruptor Pico. I am very satisfied from the way it works, plus it’s super quiet! Combining the sonicator with the Plant ChIP-seq kit we finally got things working. I have also decided to try the Microplex Library Prep kit, which is amazing. I have been working with other kits and I find this one efficient and very easy to use. Recently, I have tested one of the epigenetics antibody (H3K4me3) and it works very well on the plant tissue, together with the ChIP-seq kit and Bioruptor.

Thanks Diagenode for saving my PhD!

Kamila Kwasniewska, Plant Developmental Genetics, Smurfit Institute, Trinity College, Dublin
about Bioruptor® Pico sonication device, MicroPlex Library Preparation Kit v2 (12 indexes), H3K4me3 Antibody, H3K4me3 Antibody (sample size).

Our lab has used Diagenode’s Premium RRBS kit on rat brain samples. The protocol is understandable, logical, well-written and is easy to follow. It is a complicated, but ingenious kit. I found it fantastic that I could ask questions from the company, and their answers were really useful. We were able to construct a library, which we ran on BioAnalyzer, and the results looked very nice and ready to be sequenced. I would definitely recommend my colleagues to use the Premium RRBS kit from Diagenode.

Borbála Vető, Institute of Enzymology, Budapest, Hungary
about Premium RRBS kit, Premium RRBS kit.

I have extensively used the antibodies against the histone modifications H3K4me3, H3k27me3, H3K9ac, H4k8ac and H3K18ac provided by Diagenode. The high level of specificity and selectivity of these antibodies in mouse brain samples, confirmed by using several negative and positive controls run in parallel with mouse brain tissue samples, ensured successful and reproducible results. I have been a Diagenode costumer for over one year now and I am extremely satisfied with the efficiency of the Bioruptor Pico for chromatin shearing as well as all of the ChIP materials (i.e., antibodies, blocking peptides, primer pairs for qPCR) provided by this company. Many thanks.

Dr. Ermelinda Lomazzo, Institute of Physiological Chemistry, AG Prof. Beat Lutz. University Medical Center of the Johannes Gutenberg University Mainz, Germany
about Bioruptor® Pico sonication device, H3K4me3 Antibody, H3K27me3 Antibody, H3K9ac Antibody, H4K8ac Antibody - ChIP Grade, H3K18ac Antibody - ChIP-seq Grade, H3K27me3 Antibody (sample size).

There are so many ChIP-related products on the market, but I feel so lucky that I have been using the ones from Diagenode since I started my CHIP-seq project. I have used their iDeal CHIP-seq Kit for Transcription Factors and MicroPlex Library Prep Kit v2. Both of them are fantastic and very reproducible. With the very-well written protocols, you will just be home and dry. Particularly, I want to thank the technical support, who is very patient, knowledgeable and extremely helpful. I would definitely recommend my colleagues to use the CHIP products from Diagenode.

Dr Kaiyu Lei, Faculty of Medicine, Department of Surgery & Cancer, Imperial College London
about iDeal ChIP-seq kit for Transcription Factors, iDeal ChIP-seq kit for Transcription Factors, MicroPlex Library Preparation Kit v2 (12 indexes), iDeal ChIP-seq Kit for Transcription Factors, MicroPlex Library Preparation Kit v2 (12 indexes).

My group is mostly focused on epigenetic reprogramming and I have been using Diagenode products for the last 5 years. My experience with both antibodies, ChIP-Kits and the Bioruptor is nothing but positive. Diagenode products are unique for reproducibility, and this has always been a great plus for the success of my experiments.

Dr. Raffaele Teperino - Environmental Epigenetic Group - Institute of Experimental Genetics, Helmholtz Zentrum Muenchen GmbH, Germany
about Bioruptor® Pico sonication device.

I am working with the True MicroChIP & Microplex Library Preparation Kits and several histone modification antibodies like H3K27ac, H3K4me3, H3K36me3, and H3K27me3. I got always very good and reproducible results for my ChIP-seq experiments.

Andrea Thiesen, ZMB, Developmental Biology, Prof. Dr. Andrea Vortkamp´s lab, University Duisburg-Essen, Germany
about H3K27ac Antibody, H3K27ac Antibody (sample size), H3K4me3 Antibody, H3K4me3 Antibody (sample size), H3K36me3 Antibody, H3K36me3 Antibody (sample size), H3K27me3 Antibody, H3K27me3 antibody (sample size), True MicroChIP-seq Kit, MicroPlex Library Preparation Kit v2 (12 indexes), MicroPlex Library Preparation Kit v2 (12 indexes).

The Norwegian sequencing centre (NSC) is a national core facility fully equipped to handle a diverse set of sequencing projects. We see an increased interest in long read technologies, in which our PacBio Single Molecule sequencing service is important. PacBio, (or SMRT) sequencing, like any other single molecule system, starts with a critical library preparation step to generate long fragment libraries of 10-20kb length. Even longer libraries can be made, but common to all library types is to start with good quality DNA of high molecular weight. Physical shearing of the DNA, targeting the desired library length, is the first critical step in the procedure. NSC has experience with different methods to fragment DNA like nebulization, enzymatic, tagmentation, covaris AFA, g-tubes (also covaris), custom syringe/needle protocols and hydroshearing. The best fragmentation method should produce a sharp fragmentation pattern close to the target length as defined by the protocol settings, with as little “smear like” pattern of lower molecular length as possible. The final step of PacBio library preparation is to size select only the longest fragments (> 7kb) as this increases the overall success rate of only sequencing the longest library fragments. It is not uncommon to loose a lot of material during this step. However, if the initial fragmentation yields sharp fragmentation patterns with minimal smear, more of the total DNA is retained in the final library. This is perhaps most important on samples were DNA amounts are limited. The optimal fragmentation method has to produce similar fragmentation pattern each time, with little deviation with sample type and input amount. We have found the new Megaruptor from Diagenode to be one of the best instruments for routine shearing of DNA to lengths of 2-40kb. It is easy to use, with walk away shearing, and the instrument does all the washes before, in between samples and after shearing without user intervention. Disposable hydropores is beneficial for lowering the contamination risk. The fact that it is so easy to use make it a good choice for laboratories with multiple users and/or when training new staff.

Morten Skage and Dr Ave Tooming-Klunderud, Norwegian Sequencing Centre, Oslo, Norway
about Megaruptor®.

I am working with Diagenode's antibodies for over 5 years now. The great and specific performance as well as the reliable high quality of the antibodies are crucial for the success of my ChIP experiments.

Dr Holger Bierhoff, Division Molecular Biology of the Cell II, Prof. Dr. Ingrid Grummt’s lab, DKFZ, Heidelberg, Germany


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