The Diagenode TDG recombinant protein (Cat. No. C23020101) is a very good tool. I used it as positive control in western blot analysis and during TDG ELISA activity assays. The protein is really active! Indeed the TDG recombinant protein perfectly works in glycosylase activity assays using marked oligos.

Dr Francesco Spallotta, Division of Cardiovascular Epigenetics, Goethe University, Frankfurt am Main, Germany
about Recombinant mouse TDG .

Diagenode Ago monoclonal antibody (Cat No. C15200167) immunoprecipitates the predicted miRNA target alleles very efficiently

Haruko Takeda, University of Liège, Belgium
about Ago (Argonautes) monoclonal antibody - Classic, Ago (Argonautes) monoclonal antibody - Classic, Ago (Argonautes) monoclonal antibody - Classic (sample size).

In life sciences, epigenetics is nowadays the most rapid developing field with new astonishing discoveries made every day. To keep pace with this field, we are in need of reliable tools to foster our research - tools Diagenode provides us with. From antibodies to automated solutions - all from one source and with robust support. Antibodies used in our lab: H3K27me3 polyclonal antibody – Premium, H3K4me3 polyclonal antibody – Premium, H3K9me3 polyclonal antibody – Premium, H3K4me1 polyclonal antibody – Premium, CTCF polyclonal antibody – Classic, Rabbit IgG.

Dr. Florian Uhle, Dept. of Anesthesiology, Heidelberg University Hospital, Germany
about H3K27me3 polyclonal antibody - Premium, H3K27me3 polyclonal antibody - Premium (sample size), H3K4me3 polyclonal antibody - Premium, H3K4me3 polyclonal antibody - Premium (sample size), H3K9me3 polyclonal antibody - Premium, H3K9me3 polyclonal antibody - Premium (sample size), H3K4me1 polyclonal antibody - Premium (sample size), H3K4me1 polyclonal antibody - Premium, CTCF polyclonal antibody - Classic, CTCF polyclonal antibody - Classic (sample size), Rabbit IgG, SX-8G IP-Star® Compact Automated System.

Using the Premium RRBS kit we quickly established the RRBS library preparation in our group. Diagenode's support on wet-lab matters as well as on bioinformatics was exceptionally customer-oriented and close, accelerating the establishment of the method.

Dr. Eduard Resch, Fraunhofer Insitute for Molecular Biology and Applied Ecology IME, Project Group Translational Medicine & Pharmacology TMP, Frankfurt am Main
about Premium Reduced Representation Bisulfite Sequencing (RRBS) kit, Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit.

We use the Bioruptor sonication device and the antibody against the histone modification H3K4me3 in our lab. The Bioruptor device is used for the shearing of chromatin in ChIP-Seq experiments and for generation of protein lysates (whole cell extracts). Thanks to the Bioruptor device we achieve excellent and reproducible results in both applications. The H3K4me3 antibody is used in our ChIP-Seq experiments as well as Western Blotting. With this antibody we are able to generate highly enriched ChIP-Seq samples with extremely low background. In Western Blot we can detect one specific, strong band with the H3K4me3 antibody.

Diagenode provides not only very good products for research but also an excellent customer support.

Dr Lora Dimitrova, Charité-Universitätsmedizin Berlin, Germany
about Bioruptor® Plus sonication device, H3K4me3 polyclonal antibody - Premium, H3K4me3 polyclonal antibody - Premium (sample size).

I have been a Diagenode customer for over two years now. I have used the antibodies against the histone modifications H3K4me3, H3K4me2, H3K4me1, H3k27me3, H3K9ac, H3K27ac etc. provided by Diagenode to perform western blots.The high level of specificity of these antibodies in Pristionchus pacificus samples, confirmed by using several negative and positive controls run in parallel with synchronized culture samples, ensured successful and reproducible results.

Also, I am satisfied with the service and the support of personnel of Diagenode. They are prompt in replying to my emails and very helpful with their advice. Thanks a lot!

Vahan Serobyan, Prof. Dr Ralf J. Sommer's Lab, Max Planck Institute for Developmental Biology, Tübingen, Germany

I work with Diagenode’s Plant ChIP-seq kit and shear the DNA on the Bioruptor Pico for the last year and I have to say that these two products saved my PhD project! Some time ago, our well-established ChIP protocol suddenly stopped to work and after long time of figuring out the reason, we invested into Bioruptor Pico. I am very satisfied from the way it works, plus it’s super quiet! Combining the sonicator with the Plant ChIP-seq kit we finally got things working. I have also decided to try the Microplex Library Prep kit, which is amazing. I have been working with other kits and I find this one efficient and very easy to use. Recently, I have tested one of the epigenetics antibody (H3K4me3) and it works very well on the plant tissue, together with the ChIP-seq kit and Bioruptor.

Thanks Diagenode for saving my PhD!

Kamila Kwasniewska, Plant Developmental Genetics, Smurfit Institute, Trinity College, Dublin
about Picoruptor ® Sonicator, MicroPlex Library Preparation Kit v2 (12 indexes), H3K4me3 polyclonal antibody - Premium, H3K4me3 polyclonal antibody - Premium (sample size).

Our lab has used Diagenode’s Premium RRBS kit on rat brain samples. The protocol is understandable, logical, well-written and is easy to follow. It is a complicated, but ingenious kit. I found it fantastic that I could ask questions from the company, and their answers were really useful. We were able to construct a library, which we ran on BioAnalyzer, and the results looked very nice and ready to be sequenced. I would definitely recommend my colleagues to use the Premium RRBS kit from Diagenode.

Borbála Vető, Institute of Enzymology, Budapest, Hungary
about Premium Reduced Representation Bisulfite Sequencing (RRBS) kit, Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit.

I have extensively used the antibodies against the histone modifications H3K4me3, H3k27me3, H3K9ac, H4k8ac and H3K18ac provided by Diagenode. The high level of specificity and selectivity of these antibodies in mouse brain samples, confirmed by using several negative and positive controls run in parallel with mouse brain tissue samples, ensured successful and reproducible results. I have been a Diagenode costumer for over one year now and I am extremely satisfied with the efficiency of the Bioruptor Pico for chromatin shearing as well as all of the ChIP materials (i.e., antibodies, blocking peptides, primer pairs for qPCR) provided by this company. Many thanks.

Dr. Ermelinda Lomazzo, Institute of Physiological Chemistry, AG Prof. Beat Lutz. University Medical Center of the Johannes Gutenberg University Mainz, Germany
about Picoruptor ® Sonicator, H3K4me3 monoclonal antibody - Classic, H3K27me3 polyclonal antibody, H3K9ac polyclonal antibody - Classic, H4K8ac polyclonal antibody - Classic, H3K18ac polyclonal antibody - Classic, H3K27me3 polyclonal antibody .

There are so many ChIP-related products on the market, but I feel so lucky that I have been using the ones from Diagenode since I started my CHIP-seq project. I have used their iDeal CHIP-seq Kit for Transcription Factors and MicroPlex Library Prep Kit v2. Both of them are fantastic and very reproducible. With the very-well written protocols, you will just be home and dry. Particularly, I want to thank the technical support, who is very patient, knowledgeable and extremely helpful. I would definitely recommend my colleagues to use the CHIP products from Diagenode.

Dr Kaiyu Lei, Faculty of Medicine, Department of Surgery & Cancer, Imperial College London
about iDeal ChIP-seq kit for Transcription Factors, iDeal ChIP-seq kit for Transcription Factors, MicroPlex Library Preparation Kit v2 (12 indexes), MicroPlex Library Preparation Kit v2 (48 indexes), iDeal ChIP-seq Kit for Transcription Factors, MicroPlex Library Preparation Kit v2 (12 indexes).

My group is mostly focused on epigenetic reprogramming and I have been using Diagenode products for the last 5 years. My experience with both antibodies, ChIP-Kits and the Bioruptor is nothing but positive. Diagenode products are unique for reproducibility, and this has always been a great plus for the success of my experiments.

Dr. Raffaele Teperino - Environmental Epigenetic Group - Institute of Experimental Genetics, Helmholtz Zentrum Muenchen GmbH, Germany
about Picoruptor ® Sonicator.

The Norwegian sequencing centre (NSC) is a national core facility fully equipped to handle a diverse set of sequencing projects. We see an increased interest in long read technologies, in which our PacBio Single Molecule sequencing service is important. PacBio, (or SMRT) sequencing, like any other single molecule system, starts with a critical library preparation step to generate long fragment libraries of 10-20kb length. Even longer libraries can be made, but common to all library types is to start with good quality DNA of high molecular weight. Physical shearing of the DNA, targeting the desired library length, is the first critical step in the procedure. NSC has experience with different methods to fragment DNA like nebulization, enzymatic, tagmentation, covaris AFA, g-tubes (also covaris), custom syringe/needle protocols and hydroshearing. The best fragmentation method should produce a sharp fragmentation pattern close to the target length as defined by the protocol settings, with as little “smear like” pattern of lower molecular length as possible. The final step of PacBio library preparation is to size select only the longest fragments (> 7kb) as this increases the overall success rate of only sequencing the longest library fragments. It is not uncommon to loose a lot of material during this step. However, if the initial fragmentation yields sharp fragmentation patterns with minimal smear, more of the total DNA is retained in the final library. This is perhaps most important on samples were DNA amounts are limited. The optimal fragmentation method has to produce similar fragmentation pattern each time, with little deviation with sample type and input amount. We have found the new Megaruptor from Diagenode to be one of the best instruments for routine shearing of DNA to lengths of 2-40kb. It is easy to use, with walk away shearing, and the instrument does all the washes before, in between samples and after shearing without user intervention. Disposable hydropores is beneficial for lowering the contamination risk. The fact that it is so easy to use make it a good choice for laboratories with multiple users and/or when training new staff.

Morten Skage and Dr Ave Tooming-Klunderud, Norwegian Sequencing Centre, Oslo, Norway
about Megaruptor®.

I am working with Diagenode's antibodies for over 5 years now. The great and specific performance as well as the reliable high quality of the antibodies are crucial for the success of my ChIP experiments.

Dr Holger Bierhoff, Division Molecular Biology of the Cell II, Prof. Dr. Ingrid Grummt’s lab, DKFZ, Heidelberg, Germany

To expedite the larger-scale phenotyping of pollen number difference within Arabidopsis family, we have modified an existing method more suitable for our purpose of pollen counting using a Bioruptor®. We grew four plants per genotype. Three flower buds per plant were harvested and dried at 65°C overnight. We collected flower buds with mature pollen, but before the anthers were opened (flower stage 13). We did not use the first and second flowers of the inflorescence, as they tend to show developmentally immature flower morphologies. 30 μl of 5% Tween-20 is added to the dried flower and samples are sonicated using the Bioruptor® Plus with high power mode for 10 cycles (sonication cycle: 30 sec ON, 30 sec OFF) to separate pollen grains from the anthers. Then, all pollen solutions are measured with a cell counter.

Misako Yamazaki, Evolutionary and Ecological Genomics (Kentaro Shimizu group), Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Switzerland
about Bioruptor® Plus sonication device.

We will use  the Megaruptor® within our PacBio® Single Molecule Sequencing workflows, especially for the construction of very big insert  (>20kb) libraries.

Andrea Patrignani, Anna Bratus and Ralph Schlapbach, Functional Genomics Center Zurich (ETHZ/UZH), Switzerland
about Megaruptor®.

We are particularly satisfied with the Bioruptor® Pico that we have recently purchased. Since we have started using this novel machine, all experiments for our epignetics research have been much more easy to perform. This machine is really a great thing for us. The whole department has now started using this novel device.

Dr. Marco Pontoglio, Head of the Team "Expression Génique, Développement et Maladies" (EGDM) and Director of the “Development, Cancer and Reproduction”, Department Institut Cochin, Université Paris-Descartes
about Picoruptor ® Sonicator.

Diagenode has provided a Bioruptor® Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially  for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.

Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford
about Picoruptor ® Sonicator, 15 ml Picoruptor Tubes & sonication beads.

The new Bioruptor® Pico machine has reduced the amount of time spent sonicating Chromatin by a massive amount. Some protocols require quite harsh fixing conditions which meant fragmenting DNA on the old machine was taking many rounds and several times. With the new Bioruptor® Pico machine these sonications were taking just one round of 10 cycles thereby reducing the fragmentation time substantially. Following sonication, I have used the new IDeal ChIP-seq kit. This is a nice straight forward kit that if followed with an appropriate chip validated antibody gave amazing chip-seq results that worked time and again with several different transcription factors. I would recommend both kits for good, consistant chromatin work.

Dr. Karen Dawson, RNA Biology Group, Cancer Research UK Manchester Institute at the University of Manchester
about Picoruptor ® Sonicator, iDeal ChIP-seq kit for Histones, iDeal ChIP-seq kit for Transcription Factors.

We used the MicroPlex version 2 kit to generate libraries using ChIP DNA for several transcription factors and compared the results to a standard library generation protocol starting from 5ng of ChIP DNA. Even when we reduced the starting amount of DNA by 10-fold, the MicroPlex Kit produced the same high yields and quality of the libraries. As expected, the number of duplicate reads increased but 15 to 20 million unique reads were sufficient to achieve excellent enrichment data. We found that no information was lost, and the MicroPlex Kit helped produce data that was consistent with the standard protocol despite the lower input. On top of this, the MicroPlex Kit was extremely user-friendly and saved us time. The MicroPlex version 2 kit will make challenging ChIP-seq experiments that rely on very limited amount of starting material much easier with robust results.

Katia Basso, PhD, Assistant Professor, Columbia University, New York
about MicroPlex Library Preparation Kit v2 (12 indexes), MicroPlex Library Preparation Kit v2 (12 indexes), MicroPlex Library Preparation Kit v2 (48 indexes).

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