Diagenode

Plant ChIP-seq kit

Product image
Catalog Number
Format
Price
C01010150
24 rxns
$595.00

Diagenode's Plant ChIP-seq Kit is the only validated and complete solution for obtaining high quality ChIP-seq results from plant material. Specifically developed to extract and prepare chromatin from plant tissues, the kit provides high yields with excellent specificity and sensitivity.

  • Characteristics

    High enrichment and low background

    ChIP-seq figure 1

    Figure 1. ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our Premium H3K4me3 ChIP-seq grade antibody (#C15410003). Libraries were prepared with our MicroPlex Library Preparation™ kit (#C05010010) from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.

    ChIP-seq figure 2

    Figure 2. ChIP was performed on Arabidopsis thaliana (Col-0) seedlings 14 days after germination (dag) using our premium anti-H3K4me3 ChIP-seq grade antibody (#C15410003) and rabbit IgG (#C15410206). Data shown is derived from biological replicates. Sheared chromatin from 0.2 g (fw) of seedlings, 1 μg of H3K4me3 antibody and 1 μg of the negative IgG control were used per IP. Quantitative PCR was performed with the positive control FLC-ATG and the negative control FLC-Intron1 primer sets from the kit. The recovery is expressed as % of input.

    ChIP-seq figure 3

    Figure 3. ChIP was performed on Arabidopsis thaliana (Col-0) seedlings 14 days after germination (dag) using our premium anti-H3K27me3 ChIP-seq grade antibody (#C15410195) and rabbit IgG (#C15410206). Data shown is derived from biological replicates. Sheared chromatin from 0.2 g (fw) of seedlings, 2 μg of H3K27me3 antibody and 2 μg of the negative IgG control were used per IP. Quantitative PCR was performed with primer pairs for FLC-Intron1 (positive control) and Actin ATG (negative control). The recovery is expressed as % of input.

  • Testimonials

    I was using Diagenode Plant ChIP-seq kit to investigate abundance of particular histone mark in A.thaliana's seedlings. Plant ChIP-seq kit yielded in high amounts of immunoprecipitation DNA, very good relative enrichment to input and low background level. I frankly recommend it for ChIP on Plant tissues.

    Msc Pawel Mikulski, Lab of Dr. Daniel Schubert, Heinrich Heine University Düsseldorf

    The Diagenode Plant ChIP-seq kit works very well. The kit gives higher enrichment over background for the positive samples compared to our previous method. Using both the Plant ChIP-seq Kit and Diagenode’s Premium H3K9/14 ac polyclonal antibody, we performed ChIP-qPCR of H3K9/14 ac in region 3 of the TOC1 promoter. TOC1 is a circadian clock gene involved in evening loop that inhibits circadian clock genes expressed during the light phase of the day. We observed higher acetylation around TSS of TOC1 at the end of light phase in short day conditions, results that correlate with previously published data.

    Dorota Komar, Centre for Plant Biotechnology and Genomics, Madrid, Spain

    I work with Diagenode’s Plant ChIP-seq kit and shear the DNA on the Bioruptor Pico for the last year and I have to say that these two products saved my PhD project! Some time ago, our well-established ChIP protocol suddenly stopped to work and after long time of figuring out the reason, we invested into Bioruptor Pico. I am very satisfied from the way it works, plus it’s super quiet! Combining the sonicator with the Plant ChIP-seq kit we finally got things working. I have also decided to try the Microplex Library Prep kit, which is amazing. I have been working with other kits and I find this one efficient and very easy to use. Recently, I have tested one of the epigenetics antibody (H3K4me3) and it works very well on the plant tissue, together with the ChIP-seq kit and Bioruptor.

    Thanks Diagenode for saving my PhD!

    Kamila Kwasniewska, Plant Developmental Genetics, Smurfit Institute, Trinity College, Dublin
  • Applications
    ChIP-seq
    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomic researches, namely to investigate protein-DNA interaction on a ... Read more
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
  • Documents
    Plant ChIP-seq kit MANUAL
    Plant ChIP-seq Kit is the only validated and complete solution for obtaining high quality ChIP-se...
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    Successful application of novel plant ChIP-seq technology results in high quality sequencing data from Arabidopsis thaliana seedling cells APPLICATION NOTE
    In the recent years massively parallel sequencing (also known as Next Generation Sequencing or NG...
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    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
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  • Publications

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