Publications

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1474 publications found×

The Effect of Metformin and Carbohydrate-Controlled Diet onDNA Methylation and Gene Expression in the Endometrium of Womenwith Polycystic Ovary Syndrome.
Published: April, 2023

Abstract: Polycystic ovary syndrome (PCOS) is an endocrine disease associated with infertility and metabolic disorders in reproductive-aged women. In this study, we evaluated the expression of eight genes related to endometrial function and their DNA methyl...
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Bioruptor Antibody

Distinct regulation of EZH2 and its repressive H3K27me3 mark inPolyomavirus -positive and -negative Merkel cell carcinoma.
Published: April, 2023

Abstract: Merkel cell carcinoma (MCC) is an aggressive skin cancer for which Merkel cell polyomavirus (MCPyV) integration and expression of viral oncogenes small T and Large T have been identified as major oncogenic determinants. Recently, a component of th...
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PBRM1-deficient PBAF complexes target aberrant genomic loci to activatethe NF-ÎºB pathway in clear cell renal cell carcinoma.
Published: April, 2023

Abstract: PBRM1 encodes an accessory subunit of the PBAF SWI/SNF chromatin remodeller, and the inactivation of PBRM1 is a frequent event in kidney cancer. However, the impact of PBRM1 loss on chromatin remodelling is not well examined. Here we show that, in...
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Integrated analysis from multicentre studies identities RNAmethylation- related lncRNA risk stratification systems for glioma
Published: March, 2023

Abstract: Gastric cancer (GC) is the fourth leading cause of cancer death worldwide. Due to the lack of effective chemotherapy methods for advanced gastric cancer and poor prognosis, the emergence of immunotherapy has brought new hope to gastric cancer. Fur...
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Chromatin profiling identifies transcriptional readthrough as a conservedmechanism for piRNA biogenesis in mosquitoes.
Published: March, 2023

Abstract: The piRNA pathway in mosquitoes differs substantially from other model organisms, with an expanded PIWI gene family and functions in antiviral defense. Here, we define core piRNA clusters as genomic loci that show ubiquitous piRNA expression in bo...
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Antibody

Epigenetic dosage identifies two major and functionally distinct beta cells ubtypes.
Published: March, 2023

Abstract: The mechanisms that specify and stabilize cell subtypes remain poorly understood. Here, we identify two major subtypes of pancreatic Î² cells based on histone mark heterogeneity (beta HI and beta LO). Beta HI cells exhibit 4-fold higher...
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Comprehensive molecular phenotyping of -deficient gastric cancer revealspervasive epigenomic reprogramming and therapeutic opportunities.
Published: March, 2023

Abstract: OBJECTIVE: Gastric cancer (GC) is a leading cause of cancer mortality, with being the second most frequently mutated driver gene in GC. We sought to decipher -specific GC regulatory networks and examine therapeutic vulnerabilities arising from los...
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Antibody

Single substitution in H3.3G34 alters DNMT3A recruitment to causeprogressive neurodegeneration.
Published: March, 2023

Abstract: Germline histone H3.3 amino acid substitutions, including H3.3G34R/V, cause severe neurodevelopmental syndromes. To understand how these mutations impact brain development, we generated H3.3G34R/V/W knock-in mice and identified strikingly distinct...
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The RNA m5C Methylase NSUN2 Modulates Corneal EpithelialWound Healing.
Published: March, 2023

Abstract: PURPOSE: The emerging epitranscriptomics offers insights into the physiopathological roles of various RNA modifications. The RNA methylase NOP2/Sun domain family member 2 (NSUN2) catalyzes 5-methylcytosine (m5C) modification of mRNAs. However, the...
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Low affinity CTCF binding drives transcriptional regulation whereashigh affinity binding encompasses architectural functions
Published: February, 2023

Abstract: CTCF is a DNA-binding protein which plays critical roles in chromatin structure organization and transcriptional regulation; however, little is known about the functional determinants of different CTCF-binding sites (CBS). Using a conditional mous...
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Antibody iDeal ChIP-seq Kit for Transcription Factors

Targeting high glucose-induced epigenetic modifications at cardiac level:the role of SGLT2 and SGLT2 inhibitors.
Published: February, 2023

Abstract: BACKGROUND: Sodium-glucose co-transporters (SGLT) inhibitors (SGLT2i) showed many beneficial effects at the cardiovascular level. Several mechanisms of action have been identified. However, no data on their capability to act via epigenetic mechani...
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Myelodysplastic Syndrome associated TET2 mutations affect NK cellfunction and genome methylation.
Published: February, 2023

Abstract: Myelodysplastic syndromes (MDS) are clonal hematopoietic disorders, representing high risk of progression to acute myeloid leukaemia, and frequently associated to somatic mutations, notably in the epigenetic regulator TET2. Natural Killer (NK) cel...
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Antibody Premium RRBS Kit DNA Methylation Profiling (RRBS Service)

Species-specific regulation of XIST by the JPX/FTX orthologs.
Published: February, 2023

Abstract: X chromosome inactivation (XCI) is an essential process, yet it initiates with remarkable diversity in various mammalian species. XIST, the main trigger of XCI, is controlled in the mouse by an interplay of lncRNA genes (LRGs), some of which evolv...
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Noncanonical regulation of imprinted gene Igf2 by amyloid-beta 1-42 inAlzheimer's disease.
Published: February, 2023

Abstract: Reduced insulin-like growth factor 2 (IGF2) levels in Alzheimer's disease (AD) may be the mechanism relating age-related metabolic disorders to dementia. Since Igf2 is an imprinted gene, we examined age and sex differences in the relationship betw...
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Antibody

Detailed molecular and epigenetic characterization of the Pig IPECJ2and Chicken SL-29 cell lines
Published: February, 2023

Abstract: The pig IPECJ2 and chicken SL-29 cell lines are of interest because of their untransformed nature and wide use in functional studies. Molecular characterization of these cell lines is important to gain insight into possible molecular aberrations. ...
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Cotranscriptional demethylation induces global loss of H3K4me2 fromactive genes in Arabidopsis
Published: February, 2023

Abstract: Based on studies of animals and yeasts, methylation of histone H3 lysine 4 (H3K4me1/2/3, for mono-, di-, and tri-methylation, respectively) is regarded as the key epigenetic modification of transcriptionally active genes. In plants, however, H3K4m...
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Histone remodeling reflects conserved mechanisms of bovine and humanpreimplantation development.
Published: February, 2023

Abstract: How histone modifications regulate changes in gene expression during preimplantation development in any species remains poorly understood. Using CUT\&Tag to overcome limiting amounts of biological material, we profiled two activating (H3K4me3 ...
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Epigenetic regulation of plastin 3 expression by the macrosatelliteDXZ4 and the transcriptional regulator CHD4.
Published: February, 2023

Abstract: Dysregulated Plastin 3 (PLS3) levels associate with a wide range of skeletal and neuromuscular disorders and the most common types of solid and hematopoietic cancer. Most importantly, PLS3 overexpression protects against spinal muscular atrophy. D...
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Antibody iDeal ChIP-qPCR

Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes.
Published: February, 2023

Abstract: Enhancer activation serves as the main mechanism regulating signal-dependent transcriptional programs, ensuring cellular plasticity, yet central questions persist regarding their mechanism of activation. Here, by successfully mapping topoisomerase...
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Antibody

Methylation and expression of glucocorticoid receptor exon-1 variants andFKBP5 in teenage suicide-completers.
Published: February, 2023

Abstract: A dysregulated hypothalamic-pituitary-adrenal (HPA) axis has repeatedly been demonstrated to play a fundamental role in psychiatric disorders and suicide, yet the mechanisms underlying this dysregulation are not clear. Decreased expression of the ...
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MagMeDIP kit Antibody hMeDIP

Bridging biological cfDNA features and machine learning approaches.
Published: February, 2023

Abstract: Liquid biopsies (LBs), particularly using circulating tumor DNA (ctDNA), are expected to revolutionize precision oncology and blood-based cancer screening. Recent technological improvements, in combination with the ever-growing understanding of ce...
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MagMeDIP kit Antibody

Temporal modification of H3K9/14ac and H3K4me3 histone marksmediates mechano-responsive gene expression during the accommodationprocess in poplar
Published: February, 2023

Abstract: Plants can attenuate their molecular response to repetitive mechanical stimulation as a function of their mechanical history. For instance, a single bending of stem is sufficient to attenuate the gene expression in poplar plants to the subsequent ...
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Antibody Universal Plant ChIP-seq Kit

Incomplete transcriptional dosage compensation of vertebrate sexchromosomes is balanced by post-transcriptional compensation
Published: February, 2023

Abstract: Heteromorphic sex chromosomes (XY or ZW) present problems of gene dosage imbalance between the sexes, and with the autosomes. Mammalian X chromosome inactivation was long thought to imply a critical need for dosage compensation in vertebrates. How...
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Gene body DNA hydroxymethylation restricts the magnitude oftranscriptional changes during aging.
Published: February, 2023

Abstract: DNA hydroxymethylation (5hmC) is the most abundant oxidative derivative of DNA methylation (5mC) and is typically enriched at enhancers and gene bodies of transcriptionally active and tissue-specific genes. Although aberrant genomic 5hmC has been ...
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MagMeDIP kit Antibody hMeDIP

The age of bone marrow dictates the clonality of smooth muscle-derivedcells in atherosclerotic plaques.
Published: January, 2023

Abstract: Aging is the predominant risk factor for atherosclerosis, the leading cause of death. Rare smooth muscle cell (SMC) progenitors clonally expand giving rise to up to ~70\% of atherosclerotic plaque cells; however, the effect of age on SMC clonality...
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Antibody hMeDIP

DNA dioxygenases Tet2/3 regulate gene promoter accessibility andchromatin topology in lineage-specific loci to control epithelialdifferentiation.
Published: January, 2023

Abstract: Execution of lineage-specific differentiation programs requires tight coordination between many regulators including Ten-eleven translocation (TET) family enzymes, catalyzing 5-methylcytosine oxidation in DNA. Here, by using --driven ablation of g...
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Quantifying propagation of DNA methylation and hydroxymethylation withiDEMS.
Published: January, 2023

Abstract: DNA methylation is a critical epigenetic mark in mammalian cells. Many aspects of DNA methylation maintenance have been characterized; however, the exact kinetics of post-replicative methylation maintenance remain a subject of debate. Here we deve...
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Role of epigenetics in the etiology of hypospadias through penileforeskin DNA methylation alterations.
Published: January, 2023

Abstract: Abnormal penile foreskin development in hypospadias is the most frequent genital malformation in male children, which has increased dramatically in recent decades. A number of environmental factors have been shown to be associated with hypospadias...
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Gene Regulatory Interactions at Lamina-Associated Domains
Published: January, 2023

Abstract: The nuclear lamina provides a repressive chromatin environment at the nuclear periphery. However, whereas most genes in lamina-associated domains (LADs) are inactive, over ten percent reside in local euchromatic contexts and are expressed. How the...
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Antibody Microplex Library Preparation kit

Epigenomic charting and functional annotation of risk loci in renal cellcarcinoma.
Published: January, 2023

Abstract: While the mutational and transcriptional landscapes of renal cell carcinoma (RCC) are well-known, the epigenome is poorly understood. We characterize the epigenome of clear cell (ccRCC), papillary (pRCC), and chromophobe RCC (chRCC) by using ChIP-...
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