Alterations in the hepatocyte epigenetic landscape in steatosis.

Maji Ranjan K. et al.

Fatty liver disease or the accumulation of fat in the liver, has been reported to affect the global population. This comes with an increased risk for the development of fibrosis, cirrhosis, and hepatocellular carcinoma. Yet, little is known about the effects of a diet containing high fat and alcohol towards epigenetic aging, with respect to changes in transcriptional and epigenomic profiles. In this study, we took up a multi-omics approach and integrated gene expression, methylation signals, and chromatin signals to study the epigenomic effects of a high-fat and alcohol-containing diet on mouse hepatocytes. We identified four relevant gene network clusters that were associated with relevant pathways that promote steatosis. Using a machine learning approach, we predict specific transcription factors that might be responsible to modulate the functionally relevant clusters. Finally, we discover four additional CpG loci and validate aging-related differential CpG methylation. Differential CpG methylation linked to aging showed minimal overlap with altered methylation in steatosis.


Share this article

July, 2023


Products used in this publication

  • cut and tag antibody icon
    H3K27ac Antibody
  • cut and tag antibody icon
    H3K27me3 Antibody
  • ChIP-seq Grade
    H3K36me3 Antibody
  • cut and tag antibody icon
    H3K4me1 Antibody
  • cut and tag antibody icon
    H3K4me3 Antibody
  • cut and tag antibody icon
    H3K9me3 Antibody


 See all events


 See all news

The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics