Diagenode

H3K4ac polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410165
(pAb-165-050)
50 μg/34 μl
$295.00
  Bulk order

Polyclonal antibody raised in rabbit against histone H3 acetylated at lysine 4 (H3K4ac), using a KLH-conjugated synthetic peptide.

LotA482-0042
Concentration1.58 µg/µl
Species reactivityHuman, yeast
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 0.5 μg/ChIP Fig 1
ELISA 1:500 Fig 2
Dot Blotting 1:10,000 Fig 3
Western Blotting 1:500 Fig 4
Immunofluorescence 1:300 Fig 5

* Please note that the optimal antibody amount per IP should be determined by the end-user.

  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K4ac
    ChIP assays were performed using WT and H3K4R mutant S. pombe cells, the Diagenode antibody against H3K4ac (Cat. No. C15410165) and optimized primer pairs for qPCR. Sheared chromatin corresponding to 10 μg of DNA and 0.5 μg of antibody were used per ChIP experiment. QPCR was performed using primers specific for two different pericentric repeat regions and for the euchromatic adh1 gene. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA).

    ELISA

    Figure 2. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H3K4ac (Cat. No. C15410165) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the purified antibody was estimated to be 1:27,800.

    Dot blot

    Figure 3. Cross reactivity test using the Diagenode antibody directed against H3K4ac
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K4ac (Cat. No. C15410165) with peptides containing other histone H3 modifications and the unmodified H3K4 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 3 shows a high specificity of the antibody for the modification of interest.

    Western blot

    Figure 4. Western blot analysis using the Diagenode antibody directed against H3K4ac
    Histone extracts (15 μg) from HeLa cells were analysed by Western blot using the Diagenode antibody directed against H3K4ac (Cat. No. C15410165), diluted 1:500 in TBS-Tween containing 5% BSA. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right.

    Immunofluorescence

    Figure 5. Immunofluorescence with the Diagenode antibody directed against H3K4ac
    Wild type and H3K4R mutant S. pombe cells were stained with both the Diagenode antibody against H3K4ac (Cat. No. C15410165) (in red) and by Hoechst staining (in blue, left), or with the H3K4ac antibody alone (right). The antibody was used at a dilution of 1:300.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    DB
    Dot blotting Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H3K4ac pAb-165-050 DATASHEET
    Polyclonal antibody raised in rabbit against histone H3 acetylated at lysine 4 (H3K4ac), using a ...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K4ac polyclonal antibody - Classic (Diagenode Cat# C15410165 Lot# A482-0042). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    H3K4 acetylation, H3K9 acetylation and H3K27 methylation in breast tumor molecular subtypes
    Judes G et al.
    AIM: Here, we investigated how the St Gallen breast molecular subtypes displayed distinct histone H3 profiles. PATIENTS & METHODS: 192 breast tumors divided into five St Gallen molecular subtypes (luminal A, luminal B HER2-, luminal B HER2+, HER2+ and basal-like) were evaluated for their histone H3 modifica...

    Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells
    Dagdemir A et al.
    BACKGROUND/AIM: Numerous studies have shown that breast cancer and epigenetic mechanisms have a very powerful interactive relation. The MCF7 cell line, representative of luminal subtype and the MDA-MB 231 cell line representative of mesenchymal-like subtype were treated respectively with a Histone Methyl Transferas...

    Molecular and Epigenetic Biomarkers in Luminal Androgen Receptor: A Triple Negative Breast Cancer Subtype
    Judes G et al.

    Histone lysine trimethylation or acetylation can be modulated by phytoestrogen, estrogen or anti-HDAC in breast cancer cell lines.
    Dagdemir A, Durif J, Ngollo M, Bignon YJ, Bernard-Gallon D
    AIM: The isoflavones genistein, daidzein and equol (daidzein metabolite) have been reported to interact with epigenetic modifications, specifically hypermethylation of tumor suppressor genes. The objective of this study was to analyze and understand the mechanisms by which phytoestrogens act on chromatin in breast c...

    A chromodomain switch mediated by histone H3 Lys 4 acetylation regulates heterochromatin assembly.
    Xhemalce B, Kouzarides T
    Chromodomain proteins (Chp1/Chp2/Swi6/Clr4) bind to methylated H3K9 (H3K9me) and regulate pericentric heterochromatin in fission yeast. Chp1 and Clr4 (H3K9-HMT), bind transcriptionally active heterochromatin, whereas Chp2/Swi6 (HP1 homologs) are recruited during the inactive state. We show that H3K4 acetylation (H3K...

  • Related products

Events

  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
  • XXXIX Reunión anual de la Sociedad de Bioquimica y Biología Molecular de Chile
    Puerto Varas, Chile
    Sep 27-Sep 30, 2016
 See all events

Twitter feed

News

 See all news