Diagenode

Tagmentase (Tn5 transposase) – loaded

Catalog Number
Format
Price
C01070013-400
50 U / 400 µl
$3,200.00
Other format

Hologic Diagenode Tagmentase – Loaded is a highly efficient, hyperactive Tn5 transposase pre-loaded with Illumina-compatible sequencing adapters. By combining DNA cleavage and adapter insertion into a single step, it simplifies and optimizes workflows for Next-Generation Sequencing (NGS) applications. This product is perfectly suited for technologies such as ATAC-seq, ChIPmentation, genomic DNA tagmentation and other NGS methods, offering reliable performance and streamlined efficiency.

New! Standardized Unit Formulation
To ensure consistent performance across different batches, we have introduced and standardized Unit (U) formulation. This guarantees that you experience the same high-quality results with every purchase.

Tagmentase lot-to-lot consistency


Figure 1. Fingerprint plot demonstrates consistent efficiency of the Tagmentase (Tn5 transposase) - loaded across the samples and lots.
This figure shows the comparison of two standardized lots of Tagmentase (Lot A and Lot B). The fingerprint plot shows the efficiency of the Tagmentase enzyme, illustrating the cumulative distribution of read coverage across the genome. The x-axis represents the fraction of the genome, while the y-axis indicates the cumulative fraction of reads. The plot highlights the enrichment of reads in accessible chromatin regions, with a steep slope indicating high accessibility and a flatter slope representing less accessible regions. The data were normalized to account for sequencing depth and biases. Lot A and B show equivalent enrichment.

Figure 2. Volcano Plot Confirms Consistent Chromatin Accessibility Between Tagmentase (Tn5 transposase) – loaded Lots A and B.
The Volcano plot shows the differentially accessible sites in Lot-A compared to Lot-B, with the log2 fold change on the x-axis and the -log10() of the FDR (q-value) on the y-axis. Regions were considered as significantly differentially accessible when the log2 fold change > 2 and an adjusted p-value (q-value or FDR) < 0,01.


Figure 3. Heatmap around TSS demonstrates equivalent enrichment of the lot A and B of the Tagmentase (Tn5 transposase) - loaded.
The heatmap shows the read enrichment 3 kb up and downstream of the Transcription Start Site (TSS) of each gene present in the hg38 genome. A sharp peak reflects the openness of the TSS regions targeted by the Tagmentase enzyme.


Figure 4. Fragment length distribution: Lots A and B of the Tagmentase (Tn5 transposase) – loaded exhibit identical and expected fragment size profiles, confirming lot-to-lot consistency.
The figure shows the fragment size distribution profiles obtained from two standardized lots of Tagmentase (Lot A and Lot B). Both Tagmentase Lot A and Lot B exhibit identical fragment size distribution profiles, with matching peak intensity and shape. This consistency reflects high reproducibility between lots. In ATAC-seq experiments, a high-quality library is characterized by a sharp peak below 100 bp (representing nucleosome-free, open chromatin), a distinct peak around 200 bp (mono-nucleosomes), and additional peaks at ~400 bp and higher (multi-nucleosomes). The observed profiles from both lots align with this expected pattern, confirming the integrity and quality of the libraries.


Figure 5: IGV snapshots demonstrated identical peaks identified with two lots of standardized Tagmentase (Tn5 transposase) - loaded.
The figure shows results obtained from two standardized lots of Tagmentase (lot A and lot B). Genome browser images depict the two most intense consensus peaks across all samples, with counts per million -normalized data adjusted to the same scale for comparison.

Additional Items You May Need:

Looking for an unloaded Tagmentase? Check out Tagmentase (Tn5 transposase) – unloaded

Learn more about Tagmentation.

TESTIMONIAL

We have been using the Hyperactive Tagmentase for 2 years and its performance is outstanding - short operation time and good reproducibility, outmatching the competition. Moreover the interaction with customer representatives is always top-notch - highly efficient and knowledgeable. I can't recommend enough!

Julia Liz Touza, AstraZeneca Gothenburg, Sweden
  • Product information

    Hologic Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert sequencing adapters in a single step makes it the perfect companion for next-generation sequencing experiments. The Tagmentase is pre-loaded with sequencing adapters compatible with Illumina Nextera platforms, as shown below. The oligos loaded on the Tagmentase are inserted into DNA upon a tagmentation reaction.


    Mosaic end_reverse: 5’ [PHO]CTGTCTCTTATACACATCT 3’
    Mosaic end_Adapter A: 5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG 3’
    Mosaic end_Adapter B: 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 3’

    Underlined regions correspond to the double-stranded part of the adapter recognized by the Tagmentase.
    The final libraries can be amplified using Hologic Diagenode Primer Indexes for tagmented libraries:
    • 24 UDI for tagemented libraries - Set I
    • 24 UDI for tagmented libraries - Set II
    • 24 UDI for tagmented libraries -  Set III


    Unit (U) Definition
    One unit of Tagmentase (Tn5 Transposase) – loaded is defined as the amount of enzyme required to cleave 30 ng of linearized pUC19 plasmid in 1 hour at 37 °C, generating libraries with an average fragment size below 550 bp under standard conditions.


    Storage Conditions
    • Store at -20°C.
    • Guaranteed stable for six months from the date of receipt when stored properly.


    Storage Buffer
    • Supplied in a solution containing 50% (v/v) glycerol.

    Properties & Usage
    • Magnesium Dependency: Tagmentase requires Mg²+ for activity. Avoid chelators (e.g., EDTA, EGTA) in reaction buffers.
    • pH and Temperature: The enzyme is active at pH 7.5–8 and 37–55°C.
    • Inactivation: SDS, EDTA/EGTA, or heating to 65°C will inactivate the enzyme.


    Recommended Buffers
    Tagmentase Dilution Buffer - Hologic Diagenode, Cat. No. C01070011
    Tagmentation Buffer (2x) - Hologic Diagenode, Cat. No. C01019043 - dilute 2x before use


    Applications
    Tagmentase (Tn5 transposase) - loaded can be used in a wide range of applications to create libraries for next-generation sequencing. Recommended amounts per reaction are as follows:


    Genomic DNA tagmentation: 0.25–1 U per 25–100 ng of DNA
    ATAC-seq: 0.3 U per 50,000 cells
    ChIPmentation: 0.125 U per reaction


    Please note that additional optimization, including enzyme dose- and time-response experiments, may be required for custom protocols.


    Recommended Protocols
    For ATAC-seq and ChIPmentation, we recommend using validated Hologic Diagenode protocols:
    ATAC-seq Kit - Hologic Diagenode, Cat. No. C01080002
    ChIPmentation Kit for Histones - Hologic Diagenode, Cat. No. C01011009
    μChIPmentation Kit for Histones - Hologic Diagenode, Cat. No. C01011011
    TAG Kit for ChIPmentation - Hologic Diagenode, Cat. No. C01011030


    Quality Control
    Each new lot of Tagmentase undergoes comprehensive quality control to ensure it meets designated specifications. The following assays are performed:
    • Protein Purity and Integrity by SDS-PAGE
    • Nuclease Activity to confirm the absence of nonspecific DNase activity
    • Enzymatic Transposase Activity using a pUC19 cleavage assay and associated library preparation
    • Functional by ATAC-seq, including checks for contaminating DNA from E. coli


    Precautions
    This product is for research use only. It is not intended for use in diagnostic or therapeutic procedures.

  • Genomic DNA tagmentation protocol

    Tagmentase (Tn5 transposase) is fully compatible with genomic DNA tagmentation. We recommand using our validated protocol for optimal results. Fill out the form to access the protocol:

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