Diagenode

Antibodies

DNA modifications

The pattern of DNA modifications is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the DNA methyltransferases DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for de novo DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the Ten-Eleven Translocation (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine (5-hmC), and further into 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by Thymine DNA Glycosylase (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.

DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.

Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:

Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).

Check out the list below to see all proposed antibodies for DNA modifications.

Diagenode’s highly validated antibodies:

  • Highly sensitive and specific
  • Cost-effective (requires less antibody per reaction)
  • Batch-specific data is available on the website
  • Expert technical support
  • Sample sizes available
  • 100% satisfaction guarantee

Products

Cat. No.ProductApplicationPurity
C15200003 5-methylcytosine (5-mC) monoclonal antibody for ICC/IF - Classic
(50 μg/21 μl)
DB Epigenetics DNA Methylation FISH IF Protein A purified
C15410209 3-methylcytosine (3-mC) polyclonal antibody - Pioneer
(50 μg/28 μl)
DB Protein G purified
C15410204-100 5-Carboxylcytosine (5-caC) polyclonal antibody - Classic
(100 µg)
DB IF Methylated DNA immunoprecipitation Affinity purified
C15310200 5-formylcytosine (5-fC) polyclonal antibody - Classic
(100 µl)
DIP ELISA Whole antiserum
C15200200-50 5-hydroxymethylcytosine (5-hmC) monoclonal antibody (mouse) - Classic
(50 µg/50 µl)
DB ELISA Epigenetics DNA Methylation Protein G purified
C15220001-50 5-hydroxymethylcytosine (5-hmC) monoclonal antibody (rat) - Classic
(50 µg/32 µl)
DB ELISA Epigenetics DNA Methylation Affinity purified
C15410205-50 5-hydroxymethylcytosine (5-hmC) polyclonal antibody (rabbit) - Classic
(50 μg)
DB ELISA Methylated DNA immunoprecipitation Affinity purified
C15310210-100 5-hydroxymethylcytosine (5-hmC) polyclonal antibody (rabbit) - Classic
(100 µl)
DB ELISA Epigenetics DNA Methylation Whole antiserum
C15200081-100 5-methylcytosine (5-mC) monoclonal antibody 33D3 - Premium
(100 µg/77 µl)
DB IF Methylated DNA immunoprecipitation Protein A purified
C15200006-100 5-methylcytosine (5-mC) monoclonal antibody cl. b - Classic
(100 µg/50 µl)
IF Methylated DNA immunoprecipitation Purified by gel filtration
C15410227-100 DNMT1 polyclonal antibody - Classic
(100 μl)
IF IHC IP WB Affinity purified
C15310111 DNMT1 polyclonal antibody - Pioneer
(100 µl)
ELISA WB Whole antiserum
C15410084 DNMT3A polyclonal antibody - Classic
(50 µg/67 µl)
ELISA IP WB Affinity purified
C15410085 DNMT3A polyclonal antibody - Classic
(50 µg/54 µl)
ELISA IP WB Affinity purified
C15410086 DNMT3A polyclonal antibody - Classic
(50 µg/64 µl)
ELISA IP WB Affinity purified
C15310107 DNMT3A polyclonal antibody - Pioneer
(100 µl)
ELISA WB Whole antiserum
C15410076 DNMT3B polyclonal antibody - Classic
(50 µg/50 µl)
ChIP-qPCR ELISA Affinity purified
C15410218 DNMT3B polyclonal antibody - Classic
(50 μg/ 16 μl)
ELISA IF WB Affinity purified
C15310044 DNMT3B polyclonal antibody - Pioneer
(100 µl)
ELISA WB Whole antiserum
C15200082 N6-methyladenosine (m6A) monoclonal antibody - Classic
(50 μg/ 25 μl)
DB RIP Protein A purified
C15410208 N6-methyladenosine (m6A) polyclonal antibody - Pioneer
(50 μg/42 μl)
DB Protein G purified
C15200227 TDG monoclonal antibody - Classic
(50 μg)
IF WB Affinity purified
C15200179 TET2 monoclonal antibody - Classic
(50 μg/50 μl)
IP WB Protein G purified
C15410255-100 TET2 polyclonal antibody - Classic
(100 μl)
ChIP-qPCR IF IP WB Affinity purified
C15410311 TET3 polyclonal antibody - Pioneer
(50 μg)
ELISA WB Affinity purified

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