Diagenode

TET2 monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200179
(MAb-179-050)
50 μg/50 μl
$305.00
  Bulk order

Alternative name: MDS
Monoclonal antibody raised in mouse against a recombinant protein containing the N-terminal 300 amino acids of human TET2 (tet oncogene family member 2).

Lot004
Concentration1.0 µg/µl
Species reactivityHuman, mouse
TypeMonoclonal
PurityProtein G purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 - 1:2,000 Fig 1, 2
Immunoprecipitation 5 μg/mg of RIPA lysate Fig 2
  • Validation Data

    Western Blot

    Western Blot

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against TET2
    Figure 1A. Whole cell extracts from HL60 and NB4 cells (40 μg) were analysed by Western blot using the Diagenode antibody against TET2 (Cat. No. MAb-179-050), diluted 1:2,000 (lane 1 and 2) or 1:1,000 (lane 3 and 4) in PBS containing 10% milk. The position of the protein of interest (expected MW 224 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
    Figure 1B. Western blot on mouse E14 ES cells. The antibody was used at a dilution of 1:1,000.

    Immunoprecipitation

    Figure 2. Immunoprecipitation using the Diagenode monoclonal antibody directed against TET2
    IP was performed on 250 μg HL60 RIPA cell lysate using the Diagenode antibody against TET2 (Cat. No. MAb-179-050) (lane 3) or an IgG negative control (lane 2). The samples were analysed by Western blot analysis as described above. The input sample (25 μg RIPA lysate) was used as a positive control (lane 1).

  • Target description

    TET2 (UniProt/Swiss-Prot entry Q6N021) is a methylcytosine dioxygenase that catalyzes the conversion of 5-methylcytosine to 5-hydroxymethylcytosine (5-hmC). 5-hmC has been recently discovered in mammalian DNA and is abundant in Purkinje neurons, granule cells, embryonic stem cells, and brain tissue, especially in areas that are associated with higher cognitive function. Although its precise role has still to be shown, recent studies indicate that 5-hmC plays important roles distinct from 5-mC. Early evidence suggests that 5-hmC may represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. Mutations in TET2 have been associated with myeloproliferative diseases such as essential thrombocythemia, polycythemia vera and primary myelofibrosis.

  •  Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  •  Documents
    Datasheet TET2 C15200179 DATASHEET
    Monoclonal antibody raised in mouse against a recombinant protein containing the N-terminal 300 a...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: TET2 monoclonal antibody - Classic (Diagenode Cat# C15200179 Lot# 004). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The Role of N-α-acetyltransferase 10 Protein in DNA Methylation and Genomic Imprinting
    Lee C.C. et al.
    Genomic imprinting is an allelic gene expression phenomenon primarily controlled by allele-specific DNA methylation at the imprinting control region (ICR), but the underlying mechanism remains largely unclear. N-α-acetyltransferase 10 protein (Naa10p) catalyzes N-α-acetylation of nascent proteins, and mu...

    TET2- and TDG-mediated changes are required for the acquisition of distinct histone modifications in divergent terminal differentiation of myeloid cells
    Garcia-Gomez A. et al.
    The plasticity of myeloid cells is illustrated by a diversity of functions including their role as effectors of innate immunity as macrophages (MACs) and bone remodelling as osteoclasts (OCs). TET2, a methylcytosine dioxygenase highly expressed in these cells and frequently mutated in myeloid leukemias, may be a key...

    TET2 binds the androgen receptor and loss is associated with prostate cancer
    Nickerson M.L. et al.
    Genetic alterations associated with prostate cancer (PCa) may be identified by sequencing metastatic tumour genomes to identify molecular markers at this lethal stage of disease. Previously, we characterized somatic alterations in metastatic tumours in the methylcytosine dioxygenase ten-eleven translocation 2 (TET2)...

    TET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicing.
    Marina RJ et al.
    Intragenic 5-methylcytosine and CTCF mediate opposing effects on pre-mRNA splicing: CTCF promotes inclusion of weak upstream exons through RNA polymerase II pausing, whereas 5-methylcytosine evicts CTCF, leading to exon exclusion. However, the mechanisms governing dynamic DNA methylation at CTCF-binding sites were u...

    Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics.
    Neri F, Incarnato D, Krepelova A, Rapelli S, Anselmi F, Parlato C, Medana C, Dal Bello F, Oliviero S
    Ten eleven translocation (Tet) proteins oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). 5fC and 5caC can be further excised by thymine-DNA glycosylase (Tdg). Here, we present a genome-wide approach, named methylation-assisted bisulfite seq...

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