Protocols

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• Download DNA Shearing for Bioruptor® XL
  We highly recommend to use the tube holder for 0.5/0.65 ml tubes for the Bioruptor® XL (Cat. No. XL-pack 0.5) and the corresponding Bioruptor® 0.5 ml Microtubes for DNA Shearing (Cat. No. W...
• Download DNA shearing for Next-Generation Sequencing with the Bioruptor® Pico (2013-2019), 0.2 ml Bioruptor® microtubes and the corresponding holder
  Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, among others the precise DNA fragmentation. The Diagenode Bioruptor® Pico ensures that the DNA is e...
• Download DNA shearing guide
  DNA shearing for Next-Generation Sequencing with the Bioruptor Pico
• Download DNA Shearing with Bioruptor® Pico (2013-2019) - Small Volume (10 µl)
  For DNA shearing we highly recommend to use the 0.1 ml tube holder & tube adaptors (Cat. No. B01200041) and the corresponding 0.1 ml Bioruptor® Microtubes (Cat. No. C30010015).
• Download Dot blot protocol for 5-hydroxymethylcytosine monoclonal rat antibody
   
• Download FFPE DNA extraction protocol using the Bioruptor®
  The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, providing an invaluable repository of information for genetic analysis. These samples can be analyzed for ...
• Download iDeal Library Preparation kit - IP-Star® compact
  The “iDeal Library Preparation” protocol on the IP-Star® is using the standard “Ideal Library Preparation kit” and reagents from Diagenode. The Ideal Library Preparation...
• Download iST Sample Preparation with the Bioruptor®
  The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses.
• Download Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis
  Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck Institute for Biochemistry, Martinsried, Germany are using the Bioruptor® for their research usi...
• Download Mass-Spectrometry: procedure for shotgun proteomics
  Mass Spectrometry is the preferred method for the sequencing and characterization of proteins. Shotgun proteomics analysis of cell lines and tissues relies on stringent isolation of proteins in add...
• Download MicroPlex Library Preparation kit™ v2 - IP-Star Compact
  The “MicroPlex Library Preparation” protocol on the IP-Star® is using the standard “MicroPlex Library Preparation™ kit” and reagents from Diagenode. The MicroPlex ...
• Download NEBNext^® ChIP-Seq Library Prep for Illumina - IP-Star^® Compact -
  The “NEBNext_ChIP-Seq_Illumina” protocol on the IP-Star® is using the standard “NEBNext® ChIP-Seq Library Prep for Illumina” kit and reagents from NEB®. This kit...
• Download NEBNext® ChIP-Seq Library Prep for Illumina - IP-Star Compact
  The “NEBNext ChIP-Seq Illumina” protocol on the IP-Star® is using the standard “NEBNext® ChIP-Seq Library Prep for Illumina” kit and reagents from NEB®. This kit...
• Download PCR amplicons shearing using the Bioruptor Pico
  With the Bioruptor® Pico and the former model Bioruptor® NGS it is possible to shear PCR amplicons of at least 500 bp in size. While PCR fragments bigger than 700 bp are sheared accord...
• Download Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico
  Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Effici...
• Download Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus
  Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Effici...
• Download Re-shearing of decrosslinked immunoprecipitated DNA using the Bioruptor® Pico
  ChIP-seq is a common tool used to study protein-DNA interactions in cells and tissues on a genome-wide scale. The size of the immunoprecipitated DNA fragments is a key parameter for successful libr...
• Download RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit
  Isolation of intact RNA is essential for many techniques used in gene expression analysis. Efficient disruption and homogenization of animal tissues are required to ensure high yield of RNA. Disrup...
• Download RNA Shearing for Bioruptor® NGS and Pico
  The Diagenode ACT (Adaptative Cavitation Transfer technology) process is highly reproducible, however attention must be paid to the following operating conditions to ensure best RNA shearing r...
• Download TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
  The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a nat...