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Next Generation Sequencing

DNA/RNA library preparation

Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to fragmented DNA or RNA prior to sequencing

After input DNA has been fragmented, it is end-repaired and blunt-ended. The next step is a A-tailing in which dAMP is added to the 3´ end of the blunt phosphorylated DNA fragments to prevent concatemerization and to allow the ligation of adaptors with complementary dT overhangs. In addition, barcoded adapters can be incorporated to facilitate multiplexing prior to or during amplification.

Diagenode offers a comprehensive product portfolio for library preparation:

D-Plex RNA-seq Library Preparation Kits

Diagenode’s new RNA-sequencing solutions utilize the innovative capture and amplification by tailing and switching”, a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. Learn more

ChIP-seq and DNA sequencing library preparation solutions

Our kits have been optimized for DNA library preparation used for next generation sequencing for a wide range of inputs. Using a simple three-step protocols, our kits are an optimal choice for library preparation from DNA inputs down to 50 pg. Learn more

Bioruptor Pico - short fragments

Our well-cited Bioruptor Pico is the shearing device of choice for chromatin and DNA fragmentation. Obtain uniform and tight fragment distributions  between 150bp -2kb. Learn more

Megaruptor® - long fragments

The Megaruptor is designed to shear DNA from 3kb-75kb for long-read sequencing.  Learn more

Recommended products for DNA/RNA library preparation

Cat. No.ProductFormatPrice
C05010003 24 Dual indexes for MicroPlex Kit v3 /48 rxns
Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq library prep from ChIP-derived DNA. The kit MicroPlex v3...
48 rxns $380.00
C05010004 96 Dual indexes for MicroPlex Kit v3 – Set I /96 rxns
Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq library prep from ChIP-derived DNA. The kit MicroPlex v3...
96 rxns $1,155.00
C05010005 96 Dual indexes for MicroPlex Kit v3 – Set II /96 rxns
Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq library prep from ChIP-derived DNA. The kit MicroPlex v3...
96 rxns $1,155.00
C05010006 96 Dual indexes for MicroPlex Kit v3 – Set III /96 rxns
Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq library prep from ChIP-derived DNA. The kit MicroPlex v3...
96 rxns $1,155.00
C05010007 96 Dual indexes for MicroPlex Kit v3 – Set IV /96 rxns
Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq library prep from ChIP-derived DNA. The kit MicroPlex v3...
96 rxns $1,155.00
C05030033 D-Plex mRNA-seq Kit for Illumina
Product Features An innovative technology with template switching and UMIs Ultra-sensitive methodology with low input capability down to 10 ng High ef...
24 rxns $1,365.00
C05030010 D-Plex Single Indexes for Illumina - Set A
D-Plex Single Indexes Module - Set A includes PCR primers with 24 barcodes (i7 index) for library multiplexing with the D-Plex Small...
24 rxns $295.00
C05030011 D-Plex Single Indexes for Illumina - Set B
D-Plex Single Indexes Module - Set B includes PCR primers with 24 barcodes (i7 index) for library multiplexing with the D-Plex ...
24 rxns $295.00
C05030001 D-Plex Small RNA-seq Kit for Illumina
Product Features Ultra-low input capability, down to 10 pg for small RNAs or circulating RNAs Higher library complexity providing novel and diverse tra...
24 rxns $1,295.00
C05030031 D-Plex Total RNA-seq Kit for Illumina
Product Features An innovative technology with template switching and UMIs Ultra-low input capability, down to 50 pg for total RNAs Capture the widest...
24 rxns $1,295.00
C05030021 D-Plex Unique Dual Indexes for Illumina - Set A
D-Plex Unique Dual Indexes Module - Set A includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiplexi...
24 rxns $315.00
C05030022 D-Plex Unique Dual Indexes for Illumina - Set B
D-Plex Unique Dual Indexes Module - Set B includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiplexi...
24 rxns $315.00
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact benchtop automation system is the latest innovation in epigenetics sample preparation automation, replacing the numerous manual, err...
1 unit
C02010041 MagMeDIP-seq Package V2
MeDIP-seq library prep. kit for low DNA amount

Perform MeDIP-seq experiments, i.e. methylated DNA immunoprecipitation followed by next generation sequencing, to obtain region-resolutio...
10 rxns $1,880.00
C05010001 MicroPlex Library Preparation Kit v3 /48 rxns
Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq samples and are optimized to generate DNA libraries with...
48 rxns $1,400.00
C30010014 2 ml microtubes for SX-8G IP-Star® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System.
100 pc $41.00
C05010043 CATS mRNA-seq Kit (with polyA selection) v2 x24
An updated version of this kit using D-Plex technology is available for superior performance. Discover our NEW D-Plex mRNA-seq Kit for Illumina.
24 rxns
C05010041 CATS RNA-seq Kit v2 x24
An updated version of this kit using D-Plex technology is available for superior performance. Discover our NEW D-Plex Total RNA-seq Kit for I...
24 rxns
C05010040 CATS Small RNA-seq Kit x24
An updated version of this kit using D-Plex technology is available for superior performance. Discover our NEW D-Plex Small RNA-seq Kit for Illumi...
24 rxns
C05010042 CATS Total RNA-seq Kit (with rRNA depletion) v2 x24
An updated version of this kit using D-Plex technology is available for superior performance. Discover our NEW D-Plex Total RNA-seq Kit for I...
24 rxns
C05030032 D-Plex mRNA Capture Module
Diagenode’s D-Plex mRNA Capture Module is based on oligo d(T) magnetic beads to select only poly(A)-tailed RNAs such as mRNAs and some lncRNAs. This me...
24 rxns $70.00
C05010024 iDeal DNA IP Library Preparation Kit
Llibrary preparation for MeDIP-seq

The iDeal DNA IP Library Preparation Kit has been optimized and validated for methylated DNA immunoprecipitation sequencing (MeDIP-Seq). When using 5mC-speci...
24 rxns $1,060.00
C05010020 iDeal Library Preparation Kit x24 (incl. Index Primer Set 1)
Discover our NEW iDeal DNA IP Library Preparation Kit. An updated version of this kit is available and has been fully validated for methylate...
24 rxns
C05010025 iDeal Unique Dual Indexes for Illumina - Set A
Library preparation for MeDIP-seq

iDeal Unique Dual Indexes Module - Set A includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multip...
24 rxns $395.00
C05010021 Index Primer Set 2 (iDeal Library Preparation Kit x24)
The Index Primer Set 2 (iDeal Library Preparation Kit x24) contains an additional set of 12  indexes to be used in combination with the iDeal Library Pr...
24 rxns
C02010040 MagMeDIP-seq Package
Discover our NEW MagMeDIP-seq Package V2. An updated version of this package is available for superior performance with lower DNA amount. Perform...
10 rxns
C05010012 MicroPlex Library Preparation Kit v2 (12 indexes)
Specifically optimized for ChIP-seqThe MicroPlex Library Preparation™ kit is the only kit on the market which is validated for ChIP-seq and which allow...
12 rxns $800.00

Events

  • ChIP workshop
    Diagenode
    Sep 21-Sep 22, 2021
  • DNA methylation workshop: Master MeDIP assay
    Diagenode
    Sep 30, 2021
  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
 See all events

News

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