Diagenode’s D-Plex mRNA Capture Module is based on oligo d(T) magnetic beads to select only poly(A)-tailed RNAs such as mRNAs and some lncRNAs. This method allows manual processing of several samples at a time and can be also adapted for an automated platform.
The module is using 1 μm paramagnetic beads coated with oligo d(T)25 -mers for magnetic isolation through a gentle pull-down procedure of intact, full-length mRNAs. It is recommended to use good quality RNA (RIN≥7) as template for mRNA capture. The resulting captured-mRNA sample is recommended to be used in combination with the D-Plex Total RNA-seq Kit.