Diagenode

MicroPlex Library Preparation Kit v3 /48 rxns

Catalog Number
Format
Price
C05010001
48 rxns
$1,400.00

Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq samples and are optimized to generate DNA libraries with high molecular complexity from the lowest input amounts – down to 50 pg. The kit MicroPlex v3 includes all buffers and enzymes necessary for the library preparation. For flexibility of the choice different formats of compatible primer indexes are available separately:

NEW! Unique dual indexes :

Read more about library preparation for ChIP-seq

  • Characteristics
    • 1 tube, 2 hours, 3 steps protocol
    • Input: 50 pg – 50 ng
    • Reduce potential bias - few PCR amplification cycles needed
    • High sensitivity ChIP-seq - low PCR duplication rate
    • Great multiplexing flexibility with 24 dual indexes (8 nt)
    • Validated with the IP-Star® Automated Platform

    How it works

    MicroPlex Library Preparation Kit v3 /48 rxns

    Microplex workflow - protocol with dual indexes
    An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina® NGS platforms using a fast and simple 3-step protocol

    • Read more about MicroPlex workflow

      Step 1. Template Preparation provides efficient repair of the fragmented double-stranded DNA input.

      In this step, the DNA is repaired and yields molecules with blunt ends.

      Step 2. Library Synthesis. enables ligation of MicroPlex patented stem- loop adapters.

      In the next step, stem-loop adaptors with blocked 5’ ends are ligated with high efficiency to the 5’ end of the genomic DNA, leaving a nick at the 3’ end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.

      Step 3. Library Amplification enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.

      In the final step, the 3’ ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.

      Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.

  •  Documents
    MicroPlex Library Preparation Kit v3 MANUAL
    High Performance Library Preparation for Illumina® NGS Platforms
    Download
  •  Safety sheets
    MicroPlex Library Preparation Kit v3 SDS GB en Download
    MicroPlex Library Preparation Kit v3 SDS US en Download
    MicroPlex Library Preparation Kit v3 SDS DE de Download
    MicroPlex Library Preparation Kit v3 SDS JP ja Download
    MicroPlex Library Preparation Kit v3 SDS BE nl Download
    MicroPlex Library Preparation Kit v3 SDS BE fr Download
    MicroPlex Library Preparation Kit v3 SDS FR fr Download
    MicroPlex Library Preparation Kit v3 SDS ES es Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MicroPlex Library Preparation Kit v3 /48 rxns (Diagenode Cat# C05010001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Polycomb Repressive Complex 2 and KRYPTONITE regulate pathogen-inducedprogrammed cell death in Arabidopsis.
    Dvořák Tomaštíková E. et al.
    The Polycomb Repressive Complex 2 (PRC2) is well-known for its role in controlling developmental transitions by suppressing the premature expression of key developmental regulators. Previous work revealed that PRC2 also controls the onset of senescence, a form of developmental programmed cell death (PCD) in plants. ...

    VPRBP functions downstream of the androgen receptor and OGT to restrict p53 activation in prostate cancer
    Poulose N. et al.
    Androgen receptor (AR) is a major driver of prostate cancer (PCa) initiation and progression. O-GlcNAc transferase (OGT), the enzyme that catalyses the covalent addition of UDP-N-acetylglucosamine (UDP-GlcNAc) to serine and threonine residues of proteins, is often up-regulated in PCa with its expression correlated w...

    JAZF1, A Novel p400/TIP60/NuA4 Complex Member, Regulates H2A.ZAcetylation at Regulatory Regions.
    Procida, Tara and Friedrich, Tobias and Jack, Antonia P M and Peritore,Martina and Bönisch, Clemens and Eberl, H Christian and Daus, Nadine andKletenkov, Konstantin and Nist, Andrea and Stiewe, Thorsten and Borggrefe,Tilman and Mann, Matthias and Bartk
    Histone variants differ in amino acid sequence, expression timing and genomic localization sites from canonical histones and convey unique functions to eukaryotic cells. Their tightly controlled spatial and temporal deposition into specific chromatin regions is accomplished by dedicated chaperone and/or remodeling c...

    Histone demethylase JMJD2B/KDM4B regulates transcriptional program viadistinctive epigenetic targets and protein interactors for the maintenanceof trophoblast stem cells.
    Mak, Kylie Hin-Man et al.
    Trophoblast stem cell (TSC) is crucial to the formation of placenta in mammals. Histone demethylase JMJD2 (also known as KDM4) family proteins have been previously shown to support self-renewal and differentiation of stem cells. However, their roles in the context of the trophoblast lineage remain unclear. Here, we ...

    Trans- and cis-acting effects of Firre on epigenetic features of theinactive X chromosome.
    Fang, He and Bonora, Giancarlo and Lewandowski, Jordan P and Thakur,Jitendra and Filippova, Galina N and Henikoff, Steven and Shendure, Jay andDuan, Zhijun and Rinn, John L and Deng, Xinxian and Noble, William S andDisteche, Christine M
    Firre encodes a lncRNA involved in nuclear organization. Here, we show that Firre RNA expressed from the active X chromosome maintains histone H3K27me3 enrichment on the inactive X chromosome (Xi) in somatic cells. This trans-acting effect involves SUZ12, reflecting interactions between Firre RNA and components of t...

    H2A.Z is dispensable for both basal and activated transcription inpost-mitotic mouse muscles.
    Belotti E. et al.
    While the histone variant H2A.Z is known to be required for mitosis, it is also enriched in nucleosomes surrounding the transcription start site of active promoters, implicating H2A.Z in transcription. However, evidence obtained so far mainly rely on correlational data generated in actively dividing cells. We have e...

Events

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    Jan 18-Jan 26, 2022
  • Virtual DNA Methylation - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
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    Lausanne, Switzerland
    Feb 14-Feb 15, 2022
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