Diagenode

24 UDI for MicroPlex v3 - Set II

Catalog Number
Format
Price
C05010009
48 rxns
$420.00

The 24 Unique Dual Indexes kits (UDI) for MicroPlex v3 provide combinations of unique barcodes (unique i5 and i7 indexes) where each barcode is uniquely attributed to one sample. This is an excellent tool to identify mistakes during index sequencing. A phenomenon known as index hopping can lead to misattribution of some reads to the wrong sample, particularly frequent with the NovaSeq6000. The use of Unique Dual-Indexing (UDI) is therefore highly recommended when using this sequencer.

Two sets of UDI are available:

Each set allows for multiplexing up to 24  samples.

The Unique Dual indexes kits are compatible with the Diagenode’s MicroPlex Library Preparation Kits v3.

Read more about library preparation for ChIP-seq

  • Characteristics
    • Library preparation with MicroPlex
    • 1 tube, 2 hours, 3 steps protocol
    • Input: 50 pg – 50 ng
    • Reduce potential bias - few PCR amplification cycles needed
    • High sensitivity ChIP-seq - low PCR duplication rate
    • Allow for identification of index hopping
    • Great multiplexing flexibility
    • Validated with the IP-Star Compact Automated Platform

    How it works

    Microplex workflow - protocol with unique dual indexes
    An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina® NGS platforms using a fast and simple 3-step protocol

    • Read more about MicroPlex workflow

      Step 1. Template Preparation provides efficient repair of the fragmented double-stranded DNA input.

      In this step, the DNA is repaired and yields molecules with blunt ends.

      Step 2. Library Synthesis. enables ligation of MicroPlex patented stem- loop adapters.

      In the next step, stem-loop adaptors with blocked 5’ ends are ligated with high efficiency to the 5’ end of the genomic DNA, leaving a nick at the 3’ end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.

      Step 3. Library Amplification enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.

      In the final step, the 3’ ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.

      Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.

  •  Documents
    Primer indexes for MicroPlex kit v3 MANUAL
    High Performance Library Preparation for Illumina® NGS Platforms
    Download
  •  Safety sheets
    24 UDI for MicroPlex v3 - Set II SDS US en Download
    24 UDI for MicroPlex v3 - Set II SDS GB en Download
    24 UDI for MicroPlex v3 - Set II SDS BE fr Download
    24 UDI for MicroPlex v3 - Set II SDS FR fr Download
    24 UDI for MicroPlex v3 - Set II SDS ES es Download
    24 UDI for MicroPlex v3 - Set II SDS DE de Download
    24 UDI for MicroPlex v3 - Set II SDS JP ja Download
    24 UDI for MicroPlex v3 - Set II SDS BE nl Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 24 UDI for MicroPlex v3 - Set II (Diagenode Cat# C05010009). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    A multiomic atlas of the aging hippocampus reveals molecular changes in response to environmental enrichment
    Perez R. F. at al.
    Aging involves the deterioration of organismal function, leading to the emergence of multiple pathologies. Environmental stimuli, including lifestyle, can influence the trajectory of this process and may be used as tools in the pursuit of healthy aging. To evaluate the role of epigenetic mechanisms in this context, ...

  •  Related products

Events

  • Long-Read Sequencing Meeting 2024
    Uppsala, Sweden
    Oct 21-Oct 23, 2024
  • NextGen Omics 2024
    London, UK
    Oct 23-Oct 25, 2024
  • FEBS 2024
    Budapest, Hungary
    Oct 28-Oct 31, 2024
  • 5th Danube Conference on Epigenetics
    Budapest, Hungary
    Oct 28-Oct 31, 2024
 See all events

 


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy