Diagenode

24 Dual indexes for MicroPlex Kit v3 /48 rxns

Catalog Number
Format
Price
C05010003
48 rxns
$380.00

Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq library prep from ChIP-derived DNA. The kit MicroPlex v3 has to be purchased with the compatible set of dual indexes. This set of dual indexes allows for multiplexing up to 24 samples.

Check all available sets of dual indexes:

Read more about library preparation for ChIP-seq

  • Characteristics
    • 1 tube, 2 hours, 3 steps protocol
    • Input: 50 pg – 50 ng
    • Reduce potential bias - few PCR amplification cycles needed
    • High sensitivity ChIP-seq - low PCR duplication rate
    • Great multiplexing flexibility with 24 dual indexes (8 nt)
    • Validated with the IP-Star® Automated Platform

    How it works

    Microplex workflow - protocol with dual indexes
    An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina® NGS platforms using a fast and simple 3-step protocol

    • Read more about MicroPlex workflow

      Step 1. Template Preparation provides efficient repair of the fragmented double-stranded DNA input.

      In this step, the DNA is repaired and yields molecules with blunt ends.

      Step 2. Library Synthesis. enables ligation of MicroPlex patented stem- loop adapters.

      In the next step, stem-loop adaptors with blocked 5’ ends are ligated with high efficiency to the 5’ end of the genomic DNA, leaving a nick at the 3’ end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.

      Step 3. Library Amplification enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.

      In the final step, the 3’ ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.

      Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.

  •  Applications
    DNA/RNA library preparation
    Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to fragmented DNA or RNA prior to sequencing After input DNA has been fragmented, it is end-repaired and blunt-ended. The next step is a A-tail... Read more
  •  Documents
    Dual Indexes for MicroPlex Kit v3 MANUAL
    High Performance Library Preparation for Illumina® NGS Platformsw
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 24 Dual indexes for MicroPlex Kit v3 /48 rxns (Diagenode Cat# C05010003). Click here to copy to clipboard.

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