24 Dual indexes for MicroPlex Kit v3 /48 rxns

48 rxns

Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq library prep from ChIP-derived DNA. The kit MicroPlex v3 has to be purchased with the compatible set of dual indexes. This set of dual indexes allows for multiplexing up to 24 samples.

Check all available sets of dual indexes:

Read more about library preparation for ChIP-seq

  • Characteristics
    • 1 tube, 2 hours, 3 steps protocol
    • Input: 50 pg – 50 ng
    • Reduce potential bias - few PCR amplification cycles needed
    • High sensitivity ChIP-seq - low PCR duplication rate
    • Great multiplexing flexibility
    • Validated with the IP-Star Compact Automated Platform

    How it works

    Microplex workflow - protocol with dual indexes
    An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina® NGS platforms using a fast and simple 3-step protocol

    • Read more about MicroPlex workflow

      Step 1. Template Preparation provides efficient repair of the fragmented double-stranded DNA input.

      In this step, the DNA is repaired and yields molecules with blunt ends.

      Step 2. Library Synthesis. enables ligation of MicroPlex patented stem- loop adapters.

      In the next step, stem-loop adaptors with blocked 5’ ends are ligated with high efficiency to the 5’ end of the genomic DNA, leaving a nick at the 3’ end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.

      Step 3. Library Amplification enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.

      In the final step, the 3’ ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.

      Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.

  •  文档
    Primer indexes for MicroPlex kit v3 MANUAL
    High Performance Library Preparation for Illumina® NGS Platforms
  •  Safety sheets
    24 Dual indexes for MicroPlex Kit v3 48 rxns SDS US en Download
    24 Dual indexes for MicroPlex Kit v3 48 rxns SDS GB en Download
    24 Dual indexes for MicroPlex Kit v3 48 rxns SDS BE fr Download
    24 Dual indexes for MicroPlex Kit v3 48 rxns SDS FR fr Download
    MicroPlex Library Preparation Kit v3 SDS JP ja Download
    24 Dual indexes for MicroPlex Kit v3 48 rxns SDS ES es Download
    24 Dual indexes for MicroPlex Kit v3 48 rxns SDS DE de Download
    24 Dual indexes for MicroPlex Kit v3 48 rxns SDS JP ja Download
    24 Dual indexes for MicroPlex Kit v3 48 rxns SDS BE nl Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 24 Dual indexes for MicroPlex Kit v3 /48 rxns (Diagenode Cat# C05010003). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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    The nuclear lamina provides a repressive chromatin environment at the nuclear periphery. However, whereas most genes in lamina-associated domains (LADs) are inactive, over ten percent reside in local euchromatic contexts and are expressed. How these genes are regulated and whether they are able to interact with regu...

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    Heterogeneity of neurons reprogrammed from spinal cord astrocytes by theproneural factors Ascl1 and Neurogenin2
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    Summary Astrocytes are a viable source for generating new neurons via direct conversion. However, little is known about the neurogenic cascades triggered in astrocytes from different regions of the CNS. Here, we examine the transcriptome induced by the proneural factors Ascl1 and Neurog2 in spinal cord-derived astro...

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    The LIM and SH3 domain protein 1 (Lasp1) was originally cloned from metastatic breast cancer and characterised as an adaptor molecule associated with tumourigenesis and cancer cell invasion. However, the regulation of Lasp1 and its function in the aggressive transformation of cells is unclear. Here we use integrativ...

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