hMeDIP kit x16 (monoclonal mouse antibody)

Product image
Catalog Number
16 rxns

This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcytosine (5-hmC )(20µl, 50µl, 100µl) available in the market. It includes control sequences (5-hmC, 5-mC and unmodified cytosine DNA standards) and control primer pairs (internal and external controls), to assess the effiency of your immunoprecipitation.

  • Characteristics
    • Immunoprecipiation performed with monoclonal antibody against 5-hmC
    • Including internal & external controls for QC of each reaction
      • Mouse primers against Sf1 (hydroxymethylated gene in mouse)
      • 5-hmC, 5-mC and unmethylated DNA
    • Complete magnetic bead based kit
    • All in one tube
  • Applications
    Methylated DNA immunoprecipitation
    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is ... Read more
  • Documents
    hMeDIP kit MANUAL
    The Diagenode hMeDIP kit is designed to immunoprecipitate hydroxymethylated DNA (hMethyl ...
    Exclusive Highly Specific Kits Antibodies for DNA HydroxyMethylation Studies POSTER
    5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA by two US groups (K...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: hMeDIP kit x16 (monoclonal mouse antibody) (Diagenode Cat# C02010031). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Maternal immune activation induces GAD1 and GAD2 promoter remodeling in the offspring prefrontal cortex
    Labouesse MA et al.
    Maternal infection during pregnancy increases the risk of neurodevelopmental disorders in the offspring. In addition to its influence on other neuronal systems, this early-life environmental adversity has been shown to negatively affect cortical γ-aminobutyric acid (GABA) functions in adult life, including imp...

    Hepatic DNA hydroxymethylation is site-specifically altered by chronic alcohol consumption and aging
    Tammen SA et al.
    PURPOSE: Global DNA hydroxymethylation is markedly decreased in human cancers, including hepatocellular carcinoma, which is associated with chronic alcohol consumption and aging. Because gene-specific changes in hydroxymethylcytosine may affect gene transcription, giving rise to a carcinogenic environment, we det...

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