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Explore Diagenode’s publication database. Learn how our products are used in diverse fields of research.

1425 publications found×
Aiolos regulates eosinophil migration into tissues.
Published: August, 2021
Abstract: Expression of Ikaros family transcription factor IKZF3 (Aiolos) increases during murine eosinophil lineage commitment and maturation. Herein, we investigated Aiolos expression and function in mature human and murine eosinophils. Murine eosinophils...
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Antibody
Establishment of two homozygous CRISPR interference (CRISPRi)knock-in human induced pluripotent stem cell (hiPSC) lines for titratableendogenous gene repression.
Published: August, 2021
Abstract: Using nuclease-deficient dead (d)Cas9 without enzymatic activity fused to transcriptional inhibitors (CRISPRi) allows for transcriptional interference and results in a powerful tool for the elucidation of developmental, homeostatic and disease mec...
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Antibody CRISPR
Bisection of the X chromosome disrupts the initiation of chromosome silencing during meiosis in C. elegans
Published: August, 2021
Abstract: During meiosis, gene expression is silenced in aberrantly unsynapsed chromatin and in heterogametic sex chromosomes. Initiation of sex chromosome silencing is disrupted in meiocytes with sex chromosome-autosome translocations. To determine whether...
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Antibody
Altered Chromatin States Drive Cryptic Transcription in AgingMammalian Stem Cells.
Published: August, 2021
Abstract: A repressive chromatin state featuring trimethylated lysine 36 on histone H3 (H3K36me3) and DNA methylation suppresses cryptic transcription in embryonic stem cells. Cryptic transcription is elevated with age in yeast and nematodes, and reducing i...
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Antibody Microplex Library Preparation kit True MicroChIP kit
Enhanced targeted DNA methylation of the CMV and endogenous promoterswith dCas9-DNMT3A3L entails distinct subsequent histonemodification changes in CHO cells.
Published: July, 2021
Abstract: With the emergence of new CRISPR/dCas9 tools that enable site specific modulation of DNA methylation and histone modifications, more detailed investigations of the contribution of epigenetic regulation to the precise phenotype of cells in culture,...
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Antibody iDeal ChIP-qPCR
Evidence for long-term association of virion-delivered HBV core proteinwith cccDNA independently of viral protein production
Published: July, 2021
Abstract: Background \& Aims HBV persists in the nucleus of infected hepatocytes as a covalently closed circular DNA (cccDNA) episome that constitutes the template for viral RNA and protein synthesis. Both HBx and HBc (core) viral proteins associate wit...
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Antibody
Phase separation drives aberrant chromatin looping and cancerdevelopment.
Published: July, 2021
Abstract: The development of cancer is intimately associated with genetic abnormalities that target proteins with intrinsically disordered regions (IDRs). In human haematological malignancies, recurrent chromosomal translocation of nucleoporin (NUP98 or NUP...
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Antibody
ChIP-seq protocol for sperm cells and embryos to assess environmentalimpacts and epigenetic inheritance
Published: June, 2021
Abstract: In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We de...
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Epigenetic inheritance of DNA methylation changes in fish living inhydrogen sulfide-rich springs.
Published: June, 2021
Abstract: Environmental factors can promote phenotypic variation through alterations in the epigenome and facilitate adaptation of an organism to the environment. Although hydrogen sulfide is toxic to most organisms, the fish has adapted to survive in envir...
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E2F6 initiates stable epigenetic silencing of germline genes duringembryonic development
Published: June, 2021
Abstract: In mouse development, long-term silencing by CpG island DNA methylation is specifically targeted to germline genes; however, the molecular mechanisms of this specificity remain unclear. Here, we demonstrate that the transcription factor E2F6, a me...
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Genome-wide CRISPR screen identifies protein pathways modulating tauprotein levels in neurons
Published: June, 2021
Abstract: Aggregates of hyperphosphorylated tau protein are a pathological hallmark of more than 20 distinct neurodegenerative diseases, including Alzheimer’s disease, progressive supranuclear palsy, and frontotemporal dementia. While the exact mechan...
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Environmental enrichment preserves a young DNA methylation landscape inthe aged mouse hippocampus
Published: June, 2021
Abstract: The decline of brain function during aging is associated with epigenetic changes, including DNA methylation. Lifestyle interventions can improve brain function during aging, but their influence on age-related epigenetic changes is unknown. Using g...
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Antibody Premium RRBS Kit iDeal ChIP-seq Kit for Transcription Factors
Highly recurrent epimutations in gastric cancer CpG islandmethylator phenotypes and inflammation
Published: June, 2021
Abstract: Background CIMP (CpG island methylator phenotype) is an epigenetic molecular subtype, observed in multiple malignancies and associated with the epigenetic silencing of tumor suppressors. Currently, for most cancers including gastric cancer (GC), m...
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Antibody
Epigenetic Plasticity Enables CNS-Trafficking of EBV-infectedB Lymphocytes
Published: June, 2021
Abstract: Subpopulations of B-lymphocytes traffic to different sites and organs to provide diverse and tissue-specific functions. Here, we provide evidence that epigenetic differences confer a neuroinvasive phenotype. An EBV+ B cell lymphoma cell line (M14)...
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MagMeDIP kit
LINE-1 transcription in round spermatids is associated with accretion of5-carboxylcytosine in their open reading frames
Published: June, 2021
Abstract: Chromatin of male and female gametes undergoes a number of reprogramming events during the transition from germ cell to embryonic developmental programs. Although the rearrangement of DNA methylation patterns occurring in the zygote has been exten...
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Lasp1 regulates adherens junction dynamics and fibroblast transformationin destructive arthritis
Published: June, 2021
Abstract: The LIM and SH3 domain protein 1 (Lasp1) was originally cloned from metastatic breast cancer and characterised as an adaptor molecule associated with tumourigenesis and cancer cell invasion. However, the regulation of Lasp1 and its function in the...
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Antibody IP-Star Compact Microplex Library Preparation kit
HOTAIR lncRNA promotes epithelial-mesenchymal transition byredistributing LSD1 at regulatory chromatin regions.
Published: May, 2021
Abstract: Epithelial-to-mesenchymal transition (EMT) describes the loss of epithelial traits and gain of mesenchymal traits by normal cells during development and by neoplastic cells during cancer metastasis. The long noncoding RNA HOTAIR triggers EMT, in p...
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The lncRNA and the transcription factor WRKY42 target common cell wallEXTENSIN encoding genes to trigger root hair cell elongation.
Published: May, 2021
Abstract: Plant long noncoding RNAs (lncRNAs) are key chromatin dynamics regulators, directing the transcriptional programs driving a wide variety of developmental outputs. Recently, we uncovered how the lncRNA () directly recognizes the locus encoding the ...
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Placental uptake and metabolism of 25(OH)Vitamin D determines itsactivity within the fetoplacental unit
Published: May, 2021
Abstract: Pregnancy 25-hydroxyvitamin D (25(OH)D) concentrations are associated with maternal and fetal health outcomes, but the underlying mechanisms have not been elucidated. Using physiological human placental perfusion approaches and intact villous expl...
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Chromatin accessibility governs the differential response of cancer and T cells to arginine starvation.
Published: May, 2021
Abstract: Depleting the microenvironment of important nutrients such as arginine is a key strategy for immune evasion by cancer cells. Many tumors overexpress arginase, but it is unclear how these cancers, but not T cells, tolerate arginine depletion. ...
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Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment.
Published: May, 2021
Abstract: Human cardiac regeneration is limited by low cardiomyocyte replicative rates and progressive polyploidization by unclear mechanisms. To study this process, we engineer a human cardiomyocyte model to track replication and polyploidization using flu...
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Antibody iDeal ChIP-seq Kit for Transcription Factors
PPARɣ drives IL-33-dependent ILC2 pro-tumoral functions.
Published: May, 2021
Abstract: Group 2 innate lymphoid cells (ILC2s) play a critical role in protection against helminths and in diverse inflammatory diseases by responding to soluble factors such as the alarmin IL-33, that is often overexpressed in cancer. Nonetheless, regulat...
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Antibody
Identification of a PadR-type regulator essential for intracellularpathogenesis of
Published: May, 2021
Abstract: Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a disease endemic to the tropics. Melioidosis manifests in various ways ranging from acute skin lesions to pneumonia and, in rare cases, infection of the central nervous system....
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Epigenomic profiling of primate lymphoblastoid cell lines reveals theevolutionary patterns of epigenetic activities in gene regulatoryarchitectures
Published: May, 2021
Abstract: Changes in the epigenetic regulation of gene expression have a central role in evolution. Here, we extensively profiled a panel of human, chimpanzee, gorilla, orangutan, and macaque lymphoblastoid cell lines (LCLs), using ChIP-seq for five histone...
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Antibody
GATA6 defines endoderm fate by controlling chromatin accessibility duringdifferentiation of human-induced pluripotent stem cells
Published: May, 2021
Abstract: SUMMARY In addition to driving specific gene expression profiles, transcriptional regulators are becoming increasingly recognized for their capacity to modulate chromatin structure. GATA6 is essential for the formation of definitive endoderm; howe...
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The SAM domain-containing protein 1 (SAMD1) acts as a repressivechromatin regulator at unmethylated CpG islands
Published: May, 2021
Abstract: CpG islands (CGIs) are key regulatory DNA elements at most promoters, but how they influence the chromatin status and transcription remains elusive. Here, we identify and characterize SAMD1 (SAM domain-containing protein 1) as an unmethylated CGI-...
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Antibody Microplex Library Preparation kit
MeCP2 controls neural stem cell fate specification throughmiR-199a-mediated inhibition of BMP-Smad signaling.
Published: May, 2021
Abstract: Rett syndrome (RTT) is a severe neurological disorder, with impaired brain development caused by mutations in MECP2; however, the underlying mechanism remains elusive. We know from previous work that MeCP2 facilitates the processing of a specific ...
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Simplification of culture conditions and feeder-free expansion of bovineembryonic stem cells
Published: May, 2021
Abstract: Bovine embryonic stem cells (bESCs) extend the lifespan of the transient pluripotent bovine inner cell mass in vitro. After years of research, derivation of stable bESCs was only recently reported. Although successful, bESC culture relies on compl...
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Androgen and glucocorticoid receptor direct distinct transcriptionalprograms by receptor-specific and shared DNA binding sites.
Published: April, 2021
Abstract: The glucocorticoid (GR) and androgen (AR) receptors execute unique functions in vivo, yet have nearly identical DNA binding specificities. To identify mechanisms that facilitate functional diversification among these transcription factor paralogs,...
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USP22 Suppresses Expression in Acute Colitis and Inflammation-AssociatedColorectal Cancer.
Published: April, 2021
Abstract: As a member of the 11-gene "death-from-cancer" gene expression signature, ubiquitin-specific protease 22 (USP22) has been considered an oncogene in various human malignancies, including colorectal cancer (CRC). We recently identified an unexpected...
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