Diagenode

CRISPR

6 publications found.

Guidelines for optimized gene knockout using CRISPR/Cas9
Published: May, 2019

Abstract: CRISPR/Cas9 technology has evolved as the most powerful approach to generate genetic models both for fundamental and preclinical research. Despite its apparent simplicity, the outcome of a genome-editing experiment can be substantially impacted by...
Read more »

Long-term evaluation of AAV-CRISPR genome editing for Duchenne muscular dystrophy.
Published: March, 2019

Abstract: Duchenne muscular dystrophy (DMD) is a monogenic disorder and a candidate for therapeutic genome editing. There have been several recent reports of genome editing in preclinical models of Duchenne muscular dystrophy, however, the long-term persist...
Read more »

One-step generation of modular CAR-T cells with AAV-Cpf1.
Published: March, 2019

Abstract: Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced fea...
Read more »

CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficiency.
Published: January, 2019

Abstract: A wide range of human diseases result from haploinsufficiency, where the function of one of the two gene copies is lost. Here, we targeted the remaining functional copy of a haploinsufficient gene using CRISPR-mediated activation (CRISPRa) in and ...
Read more »

zHSF1 modulates zper2 expression in zebrafish embryos
Published: March, 2018

Abstract: HSF1 is a transcription factor that plays a key role in circadian resetting by temperature. We have used zebrafish embryos to decipher the roles of zHsf1, heat and light on zper2 transcription in vivo. Our results show that heat shock (HS) stimula...
Read more »

RNA-Based dCas9–VP64 System Improves the Viability of Cryopreserved Mammalian Cells
Published: January, 2018

Abstract: Regenerative therapies require availability of an abundant healthy cell source which can be achieved by e±cient cryopreservation techniques. Here, we established a novel approach for improved cell cryopreservation using an mRNA-based dCas9-...
Read more »

Events

  • ASHG
    Houston, TX
    Oct 15-Oct 19, 2019
  • ddd
    dd
    Oct 18-Oct 26, 2019
  • Neuroscience 2019
    Chicago, IL
    Oct 19-Oct 23, 2019
 See all events

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics