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1524 publications found×

Interactome Networks of FOSL1 and FOSL2 in Human Th17 Cells.
Published: September, 2021

Abstract: Dysregulated function of Th17 cells has implications in immunodeficiencies and autoimmune disorders. Th17 cell differentiation is orchestrated by a complex network of transcription factors, including several members of the activator protein (AP-1)...
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Antibody

The prolyl-isomerase PIN1 is essential for nuclear Lamin-Bstructure and function and protects heterochromatin under mechanicalstress.
Published: September, 2021

Abstract: Chromatin organization plays a crucial role in tissue homeostasis. Heterochromatin relaxation and consequent unscheduled mobilization of transposable elements (TEs) are emerging as key contributors of aging and aging-related pathologies, including...
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Antibody

DOT1L O-GlcNAcylation promotes its protein stability andMLL-fusion leukemia cell proliferation.
Published: September, 2021

Abstract: Histone lysine methylation functions at the interface of the extracellular environment and intracellular gene expression. DOT1L is a versatile histone H3K79 methyltransferase with a prominent role in MLL-fusion leukemia, yet little is known about ...
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WT1 regulates HOXB9 gene expression in a bidirectional way.
Published: September, 2021

Abstract: The homeoboxB9 (HOXB9) gene is necessary for specification of the anterior-posterior body axis during embryonic development and expressed in various types of cancer. Here we show that the Wilms tumor transcription factor WT1 regulates the HOXB9 ge...
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INTS11 regulates hematopoiesis by promoting PRC2 function.
Published: September, 2021

Abstract: INTS11, the catalytic subunit of the Integrator (INT) complex, is crucial for the biogenesis of small nuclear RNAs and enhancer RNAs. However, the role of INTS11 in hematopoietic stem and progenitor cell (HSPC) biology is unknown. Here, we report ...
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Antibody Microplex Library Preparation kit

Histone deacetylase 4 deletion broadly affects cardiac epigeneticrepression and regulates transcriptional susceptibility via H3K9methylation.
Published: September, 2021

Abstract: Histone deacetylase 4 (HDAC4) is a member of class IIa histone deacetylases (class IIa HDACs) and is believed to possess a low intrinsic deacetylase activity. However, HDAC4 sufficiently represses distinct transcription factors (TFs) such as the m...
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IP-Star Antibody

Genome-wide and sister chromatid-resolved profiling of protein occupancyin replicated chromatin with ChOR-seq and SCAR-seq
Published: September, 2021

Abstract: Elucidating the mechanisms underlying chromatin maintenance upon genome replication is critical for the understanding of how gene expression programs and cell identity are preserved across cell divisions. Here, we describe two recently developed t...
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Antibody

Epigenetic remodelling of enhancers in response to estrogen deprivationand re-stimulation.
Published: September, 2021

Abstract: Estrogen hormones are implicated in a majority of breast cancers and estrogen receptor alpha (ER), the main nuclear factor mediating estrogen signaling, orchestrates a complex molecular circuitry that is not yet fully elucidated. Here, we investig...
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Antibody iDeal ChIP-seq Kit for Histones iDeal ChIP-qPCR hMeDIP

Activated Histone Acetyltransferase p300/CBP-Related SignallingPathways Mediate Up-Regulation of NADPH Oxidase,Inflammation, and Fibrosis in Diabetic Kidney
Published: August, 2021

Abstract: Accumulating evidence implicates the histone acetylation-based epigenetic mechanisms in the pathoetiology of diabetes-associated micro-/macrovascular complications. Diabetic kidney disease (DKD) is a progressive chronic inflammatory microvascular ...
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Antibody

Aiolos regulates eosinophil migration into tissues.
Published: August, 2021

Abstract: Expression of Ikaros family transcription factor IKZF3 (Aiolos) increases during murine eosinophil lineage commitment and maturation. Herein, we investigated Aiolos expression and function in mature human and murine eosinophils. Murine eosinophils...
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Establishment of two homozygous CRISPR interference (CRISPRi)knock-in human induced pluripotent stem cell (hiPSC) lines for titratableendogenous gene repression.
Published: August, 2021

Abstract: Using nuclease-deficient dead (d)Cas9 without enzymatic activity fused to transcriptional inhibitors (CRISPRi) allows for transcriptional interference and results in a powerful tool for the elucidation of developmental, homeostatic and disease mec...
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Antibody CRISPR

Bisection of the X chromosome disrupts the initiation of chromosome silencing during meiosis in C. elegans
Published: August, 2021

Abstract: During meiosis, gene expression is silenced in aberrantly unsynapsed chromatin and in heterogametic sex chromosomes. Initiation of sex chromosome silencing is disrupted in meiocytes with sex chromosome-autosome translocations. To determine whether...
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Antibody

Altered Chromatin States Drive Cryptic Transcription in AgingMammalian Stem Cells.
Published: August, 2021

Abstract: A repressive chromatin state featuring trimethylated lysine 36 on histone H3 (H3K36me3) and DNA methylation suppresses cryptic transcription in embryonic stem cells. Cryptic transcription is elevated with age in yeast and nematodes, and reducing i...
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Antibody Microplex Library Preparation kit True MicroChIP kit

Phase separation drives aberrant chromatin looping and cancerdevelopment.
Published: July, 2021

Abstract: The development of cancer is intimately associated with genetic abnormalities that target proteins with intrinsically disordered regions (IDRs). In human haematological malignancies, recurrent chromosomal translocation of nucleoporin (NUP98 or NUP...
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Antibody

Enhanced targeted DNA methylation of the CMV and endogenous promoterswith dCas9-DNMT3A3L entails distinct subsequent histonemodification changes in CHO cells.
Published: July, 2021

Abstract: With the emergence of new CRISPR/dCas9 tools that enable site specific modulation of DNA methylation and histone modifications, more detailed investigations of the contribution of epigenetic regulation to the precise phenotype of cells in culture,...
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Antibody iDeal ChIP-qPCR

Evidence for long-term association of virion-delivered HBV core proteinwith cccDNA independently of viral protein production
Published: July, 2021

Abstract: Background \& Aims HBV persists in the nucleus of infected hepatocytes as a covalently closed circular DNA (cccDNA) episome that constitutes the template for viral RNA and protein synthesis. Both HBx and HBc (core) viral proteins associate wit...
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Antibody

ChIP-seq protocol for sperm cells and embryos to assess environmentalimpacts and epigenetic inheritance
Published: June, 2021

Abstract: In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We de...
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Antibody

Epigenetic inheritance of DNA methylation changes in fish living inhydrogen sulfide-rich springs.
Published: June, 2021

Abstract: Environmental factors can promote phenotypic variation through alterations in the epigenome and facilitate adaptation of an organism to the environment. Although hydrogen sulfide is toxic to most organisms, the fish has adapted to survive in envir...
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E2F6 initiates stable epigenetic silencing of germline genes duringembryonic development
Published: June, 2021

Abstract: In mouse development, long-term silencing by CpG island DNA methylation is specifically targeted to germline genes; however, the molecular mechanisms of this specificity remain unclear. Here, we demonstrate that the transcription factor E2F6, a me...
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Genome-wide CRISPR screen identifies protein pathways modulating tauprotein levels in neurons
Published: June, 2021

Abstract: Aggregates of hyperphosphorylated tau protein are a pathological hallmark of more than 20 distinct neurodegenerative diseases, including Alzheimer’s disease, progressive supranuclear palsy, and frontotemporal dementia. While the exact mechan...
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Environmental enrichment preserves a young DNA methylation landscape inthe aged mouse hippocampus
Published: June, 2021

Abstract: The decline of brain function during aging is associated with epigenetic changes, including DNA methylation. Lifestyle interventions can improve brain function during aging, but their influence on age-related epigenetic changes is unknown. Using g...
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Highly recurrent epimutations in gastric cancer CpG islandmethylator phenotypes and inflammation
Published: June, 2021

Abstract: Background CIMP (CpG island methylator phenotype) is an epigenetic molecular subtype, observed in multiple malignancies and associated with the epigenetic silencing of tumor suppressors. Currently, for most cancers including gastric cancer (GC), m...
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Antibody

Epigenetic Plasticity Enables CNS-Trafficking of EBV-infectedB Lymphocytes
Published: June, 2021

Abstract: Subpopulations of B-lymphocytes traffic to different sites and organs to provide diverse and tissue-specific functions. Here, we provide evidence that epigenetic differences confer a neuroinvasive phenotype. An EBV+ B cell lymphoma cell line (M14)...
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MagMeDIP kit

LINE-1 transcription in round spermatids is associated with accretion of5-carboxylcytosine in their open reading frames
Published: June, 2021

Abstract: Chromatin of male and female gametes undergoes a number of reprogramming events during the transition from germ cell to embryonic developmental programs. Although the rearrangement of DNA methylation patterns occurring in the zygote has been exten...
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Antibody

Lasp1 regulates adherens junction dynamics and fibroblast transformationin destructive arthritis
Published: June, 2021

Abstract: The LIM and SH3 domain protein 1 (Lasp1) was originally cloned from metastatic breast cancer and characterised as an adaptor molecule associated with tumourigenesis and cancer cell invasion. However, the regulation of Lasp1 and its function in the...
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Antibody IP-Star Compact Microplex Library Preparation kit

HOTAIR lncRNA promotes epithelial-mesenchymal transition byredistributing LSD1 at regulatory chromatin regions.
Published: May, 2021

Abstract: Epithelial-to-mesenchymal transition (EMT) describes the loss of epithelial traits and gain of mesenchymal traits by normal cells during development and by neoplastic cells during cancer metastasis. The long noncoding RNA HOTAIR triggers EMT, in p...
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The lncRNA and the transcription factor WRKY42 target common cell wallEXTENSIN encoding genes to trigger root hair cell elongation.
Published: May, 2021

Abstract: Plant long noncoding RNAs (lncRNAs) are key chromatin dynamics regulators, directing the transcriptional programs driving a wide variety of developmental outputs. Recently, we uncovered how the lncRNA () directly recognizes the locus encoding the ...
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Placental uptake and metabolism of 25(OH)Vitamin D determines itsactivity within the fetoplacental unit
Published: May, 2021

Abstract: Pregnancy 25-hydroxyvitamin D (25(OH)D) concentrations are associated with maternal and fetal health outcomes, but the underlying mechanisms have not been elucidated. Using physiological human placental perfusion approaches and intact villous expl...
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Chromatin accessibility governs the differential response of cancer and T cells to arginine starvation.
Published: May, 2021

Abstract: Depleting the microenvironment of important nutrients such as arginine is a key strategy for immune evasion by cancer cells. Many tumors overexpress arginase, but it is unclear how these cancers, but not T cells, tolerate arginine depletion. ...
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Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment.
Published: May, 2021

Abstract: Human cardiac regeneration is limited by low cardiomyocyte replicative rates and progressive polyploidization by unclear mechanisms. To study this process, we engineer a human cardiomyocyte model to track replication and polyploidization using flu...
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Antibody iDeal ChIP-seq Kit for Transcription Factors

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