Protocols
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Download Transfection of CRISPR/Cas9 Nuclease NLS ribonucleoprotein (RNP) into adherent mammalian cells using Lipofectamine® RNAiMAX
The CRISPR/Cas genome editing system consists of a single guide RNA (sgRNA) and the Cas9 endonuclease. To create gene disruptions, a single guide RNA is generated to direct the Cas9 nuclease to a s... -
Download TruSeq DNA sample preparation - IP-Star® Compact
The “TruSeq DNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeqTM DNA Sample Preparation” kit and reagents from Illumina®. It provides flexibility... -
Download TruSeqTM ChIP Sample Preparation - IP-Star® Compact -
The “TruSeq ChIP SamplePrep” protocol on the IP-Star® is using the standard “TruSeqTM ChIP Sample Preparation” kit and reagents from Illumina®. It provides flexibili... -
Download TruSeq™ ChIP Sample Preparation - IP-Star® Compact
The “TruSeq ChIP SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ ChIP Sample Preparation” kit and reagents from Illumina®. It provides flex... -
Download TruSeq™ DNA PCR-Free Sample Preparation - IP-Star Compact
The “TruSeq™ DNA PCR-Free SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ DNA PCR-Free Sample Preparation” kit and reagents from Illumina... -
Download TruSeq™ RNA Sample Preparation - IP-Star Compact
The “TruSeq RNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ RNA Sample Preparation” kit v2 and reagents from Illumina®. It provides fle... -
Download TruSeq™ Stranded RNA Sample Preparation - IP-Star® Compact
The “TruSeq Stranded RNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ Stranded Total RNA Sample Preparation” kit or “TruSeq™ Str... -
Download Ultrasensitive proteomics for microdissected fresh frozen tissue samples using the Bioruptor® Pico for sample preparation
Histology-defined proteomics investigations are a fundamental tool in modern molecular pathology. Molecular signatures of specific cells must be detected within their correct histopathological cont... -
Download Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®
The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using Bioruptor&... -
Download Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing
Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation of most archived pathological specimens. Archival FFPE samples provide an invaluable repository of in...