The “TruSeq Stranded RNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ Stranded Total RNA Sample Preparation” kit or “TruSeq™ Stranded mRNA Sample Preparation” kit and reagents from Illumina®.
It provides flexibility to prepare 1 to 16 libraries in one run starting from 1st stranded cDNA. The whole protocol takes approximately 3h30 for 8 samples. It allows you to prepare up to 32 libraries per day with 2 runs. At the end, you recover ligated products ready for size selection (if required) and amplification.