Transfection of CRISPR/Cas9 Nuclease NLS ribonucleoprotein (RNP) into adherent mammalian cells using Lipofectamine® RNAiMAX

The CRISPR/Cas genome editing system consists of a single guide RNA (sgRNA) and the Cas9 endonuclease. To create gene disruptions, a single guide RNA is generated to direct the Cas9 nuclease to a specific genomic location. Cas9-induced double strand breaks are repaired via the Non Homologous End Joining (NHEJ) DNA repair pathway. The repair is error prone, and thus insertions and deletions (INDELs) may be introduced that can disrupt gene function. Recombinant Cas9 Nuclease NLS Protein can be combined with sgRNA to form an RNP complex to be delivered to cells for rapid and highly efficient genome editing.