RRBS Service includes:
|QC of the genomic DNA||
|Preparation of RRBS libraries||
Reduced representation bisulfite sequencing (RRBS) enables genome-scale DNA methylation analysis at the single nucleotide level in any vertebrate species. The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of whole genome sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent biologically relevant target CpG-rich regions including promoters and CpG islands. Our RRBS service makes this technology widely available and provides high coverage (up to 5 million CpGs detected in human samples).
|Species||No. of uniquely aligned reads||No. of CpGs detected||No. of CpGs detected with coverage >10x||Average coverage (×)|
We are very happy with the services provided by Diagenode. In our project, Reduced Representation Bisulfite Sequencing (RRBS) was used and it worked well for both high-quality DNA and DNA extracted from formalin-fixed, paraffin-embedded (FFPE) material. Bioinformatic analyses were comprehensive and high-quality results were obtained. Diagenode’s services can be recommended.Satu Mäki-Nevala, PhD, University of Helsinki