OneDay ChIP kit

Product image
Catalog Number
60 rxns
  • Characteristics
    • 1 or 2 days from cell collection to your results (qPCR)
    • Immunoprecipitation by using agarose beads
    • Optimized purification and de-crosslinking step
    • One day from cell collection to results
    • Validated with our Shearing ChIP Kits

    The OneDay ChIP kit has been validated using chromatin sheared by sonication using the Bioruptor®. The Kit includes a DNA purifying slurry for an extra-fast method to purify your IP’d material (for qPCR analysis). Alternatively, for other applications, e.g. sequencing, linear amplification or microarray, magnetic DNA purification (IPure[Product IPure]) can be performed alternatively.

  • Applications
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
  • Documents
    OneDay ChIP kit MANUAL
    In the OneDay ChIP method the protocol has been improved to enhance the utility of the Ch...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: OneDay ChIP kit (Diagenode Cat# C01010080). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Biphasic regulation of chondrocytes by Rela through induction of anti-apoptotic and catabolic target genes
    Kobayashi H. et al.
    In vitro studies have shown that Rela/p65, a key subunit mediating NF-κB signalling, is involved in chondrogenic differentiation, cell survival and catabolic enzyme production. Here, we analyse in vivo functions of Rela in embryonic limbs and adult articular cartilage, and find that Rela protects chondrocytes ...

    Esrrb directly binds to Gata6 promoter and regulates its expression with Dax1 and Ncoa3
    Uranishi K et al.
    Estrogen-related receptor beta (Esrrb) is expressed in embryonic stem (ES) cells and is involved in self-renewal ability and pluripotency. Previously, we found that Dax1 is associated with Esrrb and represses its transcriptional activity. Further, the disruption of the Dax1-Esrrb interaction increases the expression...

    Chromatin remodeling regulates catalase expression during cancer cells adaptation to chronic oxidative stress.
    Glorieux C. et al.
    Regulation of ROS metabolism plays a major role in cellular adaptation to oxidative stress in cancer cells, but the molecular mechanism that regulates catalase, a key antioxidant enzyme responsible for conversion of hydrogen peroxide to water and oxygen, remains to be elucidated. Therefore, we investigated the trans...

    Induction of cell differentiation activates transcription of the Sarco/Endoplasmic Reticulum calcium-ATPase 3 gene (ATP2A3) in gastric and colon cancer cells
    Flores-Peredo L et al.
    The Sarco/Endoplasmic Reticulum Ca2+ -ATPases (SERCAs), pump Ca2+ into the endoplasmic reticulum lumen modulating cytosolic Ca2+ concentrations to regulate various cellular processes including cell growth. Previous studies have reported a downregulation of SERCA3 protein expression in gastric and colon cancer cell l...

    Regulatory Interaction between the Cellular Restriction Factor IFI16 and Viral pp65 (pUL83) Modulates Viral Gene Expression and IFI16 Protein Stability
    Biolatti M et al.
    A key player in the intrinsic resistance against human cytomegalovirus (HCMV) is the interferon-γ-inducible protein 16 (IFI16), which behaves as a viral DNA sensor in the first hours post infection and as a repressor of viral gene transcription in the later stages. Previous studies on HCMV replication demonstr...

    miR-125b-1 is repressed by histone modifications in breast cancer cell lines
    Cisneros-Soberanis F et al.
    PURPOSE: Downregulation of miR-125b-1 is associated with poor prognosis in breast cancer patients. In this work we investigated the effect of histone modifications on the regulation of this gene promoter. METHODS AND RESULTS: We evaluated the enrichment of two histone modifications involved in gene repression, ...

    Osterix and RUNX2 are Transcriptional Regulators of Sclerostin in Human Bone
    Flor M. Pérez-Campo, Ana Santurtún, Carmen García-Ibarbia, María A. Pascual, Carmen Valero, Carlos Garcés, Carolina Sañudo, María T. Zarrabeitia, José A. Riancho
    Sclerostin, encoded by the SOST gene, works as an inhibitor of the Wnt pathway and therefore is an important regulator of bone homeostasis. Due to its potent action as an inhibitor of bone formation, blocking sclerostin activity is the purpose of recently developed anti-osteoporotic treatments. Two bone-specific tra...

    DNMT1 and HDAC2 Cooperate to Facilitate Aberrant Promoter Methylation in Inorganic Phosphate-Induced Endothelial-Mesenchymal Transition
    Tan X et al.
    While phosphorus in the form of inorganic or organic phosphate is critically involved in most cellular functions, high plasma levels of inorganic phosphate levels have emerged as independent risk factor for cardiac fibrosis, cardiovascular morbidity and decreased life-expectancy. While the link of high phosphate and...

  • Related products


 See all events

Twitter feed


 See all news