Diagenode

iDeal ChIP-FFPE Kit

Catalog Number
Format
Price
C01010190
24 IP
$545.00

The iDeal ChIP-FFPE Kit provides a unique and robust ChIP protocol suitable for investigation of proteins associated with chromatin from FFPE samples and provides high quality yields of DNA. This solution makes traditionally difficult work with FFPE samples simpler and safer. The protocol involves easy and quick and non-toxic deparaffinization, followed by chromatin extraction and shearing by sonication. The kit contains everything you need for start-to-finish chromatin immunoprecipitation with an optimized protocol to make ChIP from FFPE samples simple yet reliable. In addition, the kit has been thorougly validated and optimized for histones and transcription factors.

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  • Characteristics
    • User-friendly, fast, and low toxicity deparaffinization workflow (unlike xylene-based methods)
    • Easy recovery of re-hydrated tissue with DiaFilter columns
    • Fast chromatin preparation without enzymatic digestion
    • Quality: Mild de-crosslinking preserves dsDNA
    • Eluted DNA suitable for qPCR analysis or other downstream applications

    Successful chromatin shearing using the Bioruptor Pico and iDeal ChIP-FFPE Kit.

    Figure 1. Sections of FFPE Mouse liver (stored for 3 years) were processed accordingly to the iDeal ChIP-FFPE protocol. The recovered sheared DNA was analysed using Fragment Analyzer (High Sensitivity NGS Fragment Analysis Kit (DNF-473). The Fragment Analyzer trace shows the fragment size distribution between 100-400 bp ideal for ChIP experiments.

    Excellent Histone ChIP results from FFPE samples

    Figure 2. Chromatin immunoprecipitation analysis using H3K27ac antibody. ChIP was performed on Mouse liver FFPE samples accordingly to the iDeal ChIP-FFPE protocol using 1 µg of H3K27ac antibody (Diagenode, Cat. no. C15410174)

    Excellent TF ChIP results from FFPE samples

    Figure 3. Chromatin immunoprecipitation analysis using CTCF antibody. ChIP was performed on Mouse liver FFPE samples accordingly to the iDeal ChIP-FFPE protocol using 1 µl of CTCF antibody (Diagenode, Cat. no. C15410210).

  • Additional solutions compatible with iDeal ChIP-FFPE Kit

    The Chromatin shearing optimization kit – Low SDS (iDeal Kit for TFs) is the kit compatible with the iDeal ChIP-FFPE kit, recommended for the optimization of chromatin shearing, a critical step for ChIP.

    Highly specific ChIP-grade antibodies, provided with batch-specific validation data.

    The DiaMag1.5 magnetic rack - for fast and efficient isolation of magnetic beads.

    Check out the list of available Primer pairs designed for high specificity to specific genomic regions.

  •  Documents
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: iDeal ChIP-FFPE Kit (Diagenode Cat# C01010190). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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