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iDeal ChIP-FFPE Kit

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24 IP

iDeal ChIP-FFPEキットは、FFPEサンプルから採取したクロマチンに関連するタンパク質の研究に適したChIPプロトコールを提供し、高品質のDNA収量を実現します。 プロトコールは従来の複雑で面倒な作業が改善され、シンプルです。無毒性脱パラフィン化と最適化されているFFPEサンプルは安全で信頼性の高い結果にやくだちます。  このキットは検証済みでヒストンと転写因子に最適化されていて、クロマチン免疫沈降に必要なものがすべて揃います。


  • 特徴
    • User-friendly, fast, and low toxicity deparaffinization workflow (unlike xylene-based methods)
    • Easy recovery of re-hydrated tissue with DiaFilter columns
    • Fast chromatin preparation without enzymatic digestion
    • Quality: Mild de-crosslinking preserves dsDNA
    • Eluted DNA suitable for qPCR analysis or other downstream applications

    Successful chromatin shearing using the Bioruptor Pico and iDeal ChIP-FFPE Kit.

    Figure 1. Sections of FFPE Mouse liver (stored for 3 years) were processed accordingly to the iDeal ChIP-FFPE protocol. The recovered sheared DNA was analysed using Fragment Analyzer (High Sensitivity NGS Fragment Analysis Kit (DNF-473). The Fragment Analyzer trace shows the fragment size distribution between 100-400 bp ideal for ChIP experiments.

    Excellent Histone ChIP results from FFPE samples

    Figure 2. Chromatin immunoprecipitation analysis using H3K27ac antibody. ChIP was performed on Mouse liver FFPE samples accordingly to the iDeal ChIP-FFPE protocol using 1 µg of H3K27ac antibody (Diagenode, Cat. no. C15410174)

    Excellent TF ChIP results from FFPE samples

    Figure 3. Chromatin immunoprecipitation analysis using CTCF antibody. ChIP was performed on Mouse liver FFPE samples accordingly to the iDeal ChIP-FFPE protocol using 1 µl of CTCF antibody (Diagenode, Cat. no. C15410210).

  •  資料
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: iDeal ChIP-FFPE Kit (Diagenode Cat# C01010190). Click here to copy to clipboard.

    Using our products in your publication? Let us know!


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    Oct 1-Oct 31, 2021



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