Library preparation for RNA sequencing

D-Plex technology is the latest innovation in RNA-sequencing. This ultrasensitive, ligation-free method utilizes the capture and amplification by tailing and switching for library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. This technology offers a new RNA-sequencing solution, optimized and specifically formatted for both Illumina and MGI sequencing platforms.

Using D-Plex technology is optimal for the study of non-coding RNAs that regulate gene expression at the transcriptional and post-transcriptional levels. The library preparation enables diverse and novel transcript detection, ensuring realistic representation of the transcriptome with minimized bias. The unique molecular identifier (UMI) sequence added in D-Plex Indexes additionally enables the accurate bioinformatic identification and removal of PCR duplicates from amplified libraries.

Our new D-Plex RNA-seq kits provide streamlined workflows with easy-to-follow protocols. The library preparation takes place in a single tube, increasing the efficiency tremendously. This ensures high technical reliability and reproducibility. Our D-Plex RNA-seq kits have been extensively validated for clinically relevant samples such as liquid biopsies or FFPE samples. The D-Plex technology is optimal for epigenetic biomarker discovery.