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NGS Library preparation

Library preparation for RNA sequencing

D-Plex Small RNA-seq library prep kit with UMI for Illumina

D-Plex technology is Diagenode’s latest innovation in RNA-sequencing. This ultrasensitive ligation-free method utilizes the innovative capture and amplification by tailing and switching for preparation of DNA libraries from extremely low input amounts, down to 10 pg for small RNA and 100 pg for total RNA. This technology offers new RNA-sequencing solution, optimized and specifically formatted for both Illumina’s and MGI’s DNBSEQTM sequencers.

Using D-Plex technology for RNA-sequencing is optimal for the study of non-coding RNA that regulate gene expression at the transcriptional and post-transcriptional levels. It enables diverse and novel transcript detection, ensuring realistic representation of the transcriptome with minimized bias. 

This new solution has been extensively validated for liquid biopsy, such as from human plasma, making it an important tool for clinically-relevant studies.

Why the new D-Plex technology benefits RNA research?

  • Innovative, ultrasensitive ligation-free method for library preparation for RNA-sequencing
  • Ultra-low input capability, down to 10 pg for small RNAs and 100 pg for total RNAs
  • High library complexity providing novel and diverse transcript detection
  • Optimal performance on clinical samples, such as liquid biopsies
  • Easy to use with minimal hands-on time: one day, one tube protocol
  • Compatible for both Illumina's and MGI's sequencing platforms

Read more about D-Plex Small RNA-seq Kit for Illumina.

Illumina sequencing platform

Cat. No.ProductFormatPrice
C05030010 D-Plex 24 Single Indexes for Illumina - Set A
D-Plex Single Indexes should be used with the D-Plex Small RNA-seq Kit. They were designed and validated to fit the D-Plex technology for Illumina seque...
24 rxns $295.00
C05030011 D-Plex 24 Single Indexes for Illumina - Set B
D-Plex Single Indexes should be used with the D-Plex Small RNA-seq Kit. They were designed and validated to fit the D-Plex technology for Illumina seque...
24 rxns $295.00
C05030001 D-Plex Small RNA-seq Kit for Illumina
Download the manual D-Plex Small RNA-seq Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the D-Plex techn...
24 rxns $1,295.00
C05010043 CATS mRNA-seq Kit (with polyA selection) v2 x24
Diagenode’s CATS mRNA-seq Kit (with polyA selection) utilizes the innovative “Capture and Amplification by Tailing and Switching” (CATS), a...
24 rxns $1,479.00
C05010041 CATS RNA-seq Kit v2 x24
Diagenode’s CATS RNA-seq Kit utilizes the innovative “Capture and Amplification by Tailing and Switching” (CATS), a ligation-free method to...
24 rxns $1,438.00
C05010040 CATS Small RNA-seq Kit x24
An updated version of this kit using D-Plex technology is available for superior performance. Discover our D-Plex Small RNA-seq kit for Illumina&n...
24 rxns
C05010042 CATS Total RNA-seq Kit (with rRNA depletion) v2 x24
Diagenode’s CATS total RNA-seq Kit (with rRNA depletion) utilizes the innovative “Capture and Amplification by Tailing and Switching” (CATS...
24 rxns

MGI sequencing platform

Cat. No.ProductFormatPrice
C05030060 D-Plex 24 DNBSEQ Barcodes for MGI - Set A
D-Plex DNBSEQ Barcodes should be used with the D-Plex Small RNA DNBSEQTM Kit. They were designed and validated to fit the D-Plex technology for MGI seque...
24 rxns $295.00
C05030061 D-Plex 24 DNBSEQ Barcodes for MGI - Set B
D-Plex DNBSEQ Barcodes should be used with the D-Plex Small RNA DNBSEQTM Kit. They were designed and validated to fit the D-Plex technology for MGI ...
24 rxns $295.00
C05030051 D-Plex Small RNA DNBSEQ™ Kit for MGI
Download the manual D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the D-...
24 rxns $1,295.00

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