Diagenode

DNMT1 polyclonal antibody - Pioneer

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310111
(CS-111-100)
100 µl
$195.00
  Bulk order

Alternative names: Dnmt, Met1, Met-1, Uim, MCMT, M.MmuI 

Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein.

LotA386-004
Concentrationnot determined
Species reactivityMouse
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:1,000 Fig 1
Western Blotting 1:500 Fig 2 (1)
  • Validation Data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DNMT1 (Cat. No. CS-111-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against DNMT1 (1)
    Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse DNMT1 (Cat. No. CS-111-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right.

  • Target description

    DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Inactivation of DNMT1 causes global demethylation and embryonic lethality. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ELISA
    Enzyme-linked immunosorbent assay. Read more
  • Documents
    Datasheet DNMT1 CS-111-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: DNMT1 polyclonal antibody - Pioneer (Diagenode Cat# C15310111 Lot# A386-004). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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