Diagenode

3-methylcytosine (3-mC) polyclonal antibody - Pioneer

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410209
50 μg/28 μl
$195.00
  Bulk order

Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA.

LotA2111-0010
Concentration1.80 µg/µl
Species reactivityHuman, mouse, other (wide range)
TypePolyclonal
PurityProtein G purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Dot Blotting 1:400 Fig 1

* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.

  • Validation Data

    Dot blot

    Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC
    To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.

  • Target description

    3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.

  • Applications
    DB
    Dot blotting Read more
  • Documents
    Datasheet 3-mC C15410209 DATASHEET
    Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 3-methylcytosine (3-mC) polyclonal antibody - Pioneer (Diagenode Cat# C15410209 Lot# A2111-0010). Click here to copy to clipboard.

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