3-methylcytosine (3-mC) polyclonal antibody - Pioneer

50 μg/28 μl
  Bulk order

Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA.

Concentration1.80 µg/µl
Species reactivityHuman, mouse, other (wide range)
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Dot Blotting 1:400 Fig 1

* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.

  • Validation Data

    Dot blot

    Figure 1. Dot blot analysis using the Diagenode antibody directed against 3-mC
    To demonstrate the specificity of the Diagenode antibody against 3-mC (cat. No. C15410209), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.

  • Target description

    3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.

  •  実験手法
    Dot blotting Read more
  •  資料
    Datasheet 3-mC C15410209 DATASHEET
    Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
  •  出版物

    How to properly cite this product in your work

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