5-methylcytosine (5-mC) monoclonal antibody 33D3

Catalog Number
500 µg
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The 5-methylcytosine antibody (clone 33D3) is the most published and widely used antibody for DNA methylation analysis. It has been validated for Methylated DNA Immunoprecipitation (MeDIP-seq, MeDIP-on-chip), Immunofluorescence and Dot blot. 

Diagenode is the exclusive worldwide source of genuine 33D3 clone!

Concentration1.1 µg/µl
Species reactivityHuman, mouse, rat, cow, other (wide range): positive
MeDIP grade.
MeDIP-seq grade.
PurityProtein A purified monoclonal antibody.
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
MeDIP/MeDIP-seq * 0.1 - 2 µg/IP Fig 1, 2
Dot Blotting ** 1:300 Fig 3
IF 1:500 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.1-5 µg per IP.

** Dot blot was only performed to demonstrate the specificity. This antibody is not recommended for dot blot on biological samples.