Diagenode

Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses.


Rebollo R, Mager DL

Endogenous retroviruses are repetitive sequences found abundantly in mammalian genomes which are capable of modulating host gene expression. Nevertheless, most endogenous retrovirus copies are under tight epigenetic control via histone-repressive modifications and DNA methylation. Here we describe a common method used in our laboratory to detect, quantify, and compare mammalian endogenous retrovirus DNA methylation. More specifically we describe methylated DNA immunoprecipitation (MeDIP) followed by quantitative PCR.

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Bioruptor
Antibody
5mC (C15200081)

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Published
February, 2016

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Products used in this publication

  • Mouse IgG
    C15200081-100
    5-methylcytosine (5-mC) monoclonal antibody 33D3
  • Mouse IgG
    C15400001-15
    Mouse IgG

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