Publications

Explore Diagenode’s publication database. Learn how our products are used in diverse fields of research.

1524 publications found×

Transcription initiation platforms and GTF recruitment at tissue-specific enhancers and promoters.
Published: August, 2011

Abstract: Recent work has shown that RNA polymerase (Pol) II can be recruited to and transcribe distal regulatory regions. Here we analyzed transcription initiation and elongation through genome-wide localization of Pol II, general transcription factors (GT...
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Antibody

5-Hydroxymethylcytosine is associated with enhancers and gene bodies in human embryonic stem cells.
Published: June, 2011

Abstract: BACKGROUND: 5-Hydroxymethylcytosine (5hmC) was recently found to be abundantly present in certain cell types, including embryonic stem cells. There is growing evidence that TET proteins, which convert 5-methylcytosine (5mC) to 5hmC, play important...
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Antibody

Early-stage formation of an epigenetic field defect in a mouse colitis model, and non-essential roles of T- and B-cells in DNA methylation induction.
Published: June, 2011

Abstract: Epigenetic fields for cancerization are involved in development of human cancers, especially those associated with inflammation and multiple occurrences. However, it is still unclear when such field defects are formed and what component of inflamm...
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Antibody

Enhancer of Zeste 2 (EZH2) is up-regulated in malignant gliomas and in glioma stem-like cells.
Published: June, 2011

Abstract: AIMS: Proteins of the Polycomb repressive complex 2 (PRC2) are epigenetic gene silencers and are involved in tumour development. Their oncogenic function might be associated with their role in stem cell maintenance. The histone methyltransferase E...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody

LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells.
Published: June, 2011

Abstract: We identify LSD1 (lysine-specific demethylase 1; also known as KDM1A and AOF2) as a key histone modifier that participates in the maintenance of pluripotency through the regulation of bivalent domains, a chromatin environment present at the regula...
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Antibody

H3.5 is a novel hominid-specific histone H3 variant that is specifically expressed in the seminiferous tubules of human testes.
Published: June, 2011

Abstract: The incorporation of histone variants into chromatin plays an important role for the establishment of particular chromatin states. Six human histone H3 variants are known to date, not counting CenH3 variants: H3.1, H3.2, H3.3 and the testis-specif...
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Antibody H3K4me3 (C15410003)

Caenorhabditis elegans chromatin-associated proteins SET-2 and ASH-2 are differentially required for histone H3 Lys 4 methylation in embryos and adult germ cells.
Published: May, 2011

Abstract: Methylation of histone H3 lysine 4 (H3K4me), a mark associated with gene activation, is mediated by SET1 and the related mixed lineage leukemia (MLL) histone methyltransferases (HMTs) across species. Mammals contain seven H3K4 HMTs, Set1A, Set1B, ...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody

Epigenetic profile of the euchromatic region of human Y chromosome.
Published: May, 2011

Abstract: The genome of a multi-cellular organism acquires various functional capabilities in different cell types by means of distinct chromatin modifications and packaging states. Acquired during early development, the cell type-specific epigenotype is ma...
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Bioruptor Chromatin Shearing Antibody LowCell ChIP kit

Regulation of mouse embryonic stem cell self-renewal by a Yes-YAP-TEAD2 signaling pathway downstream of LIF.
Published: April, 2011

Abstract: The cytoplasmic tyrosine kinase Yes has previously been shown to have an important role in maintaining mouse and human embryonic stem (ES) self-renewal through an unknown pathway downstream of leukemia inhibitory factor (LIF) and one or more facto...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody

Epigenetic switch involved in activation of pioneer factor FOXA1-dependent enhancers.
Published: April, 2011

Abstract: Transcription factors (TFs) bind specifically to discrete regions of mammalian genomes called cis-regulatory elements. Among those are enhancers, which play key roles in regulation of gene expression during development and differentiation. Despite...
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DNA shearing Bioruptor Chromatin Shearing ChIP-qPCR Antibody

Genome-wide analysis of 5-hydroxymethylcytosine distribution reveals its dual function in transcriptional regulation in mouse embryonic stem cells.
Published: April, 2011

Abstract: Recent studies have demonstrated that the Ten-eleven translocation (Tet) family proteins can enzymatically convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). While 5mC has been studied extensively, little is known about the distribu...
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Antibody

Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis.
Published: April, 2011

Abstract: Protein tagging offers many advantages for proteomic and regulomic research. Ideally, protein tagging is equivalent to having a high affinity antibody for every chosen protein. However, these advantages are compromised if the tagged protein is ove...
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Bioruptor Chromatin Shearing ChIP-qPCR Cell Lysis Western Blot Antibody

Epigenetic Regulation of Glucose Transporters in Non-Small Cell Lung Cancer
Published: March, 2011

Abstract: Due to their inherently hypoxic environment, cancer cells often resort to glycolysis, or the anaerobic breakdown of glucose to form ATP to provide for their energy needs, known as the Warburg effect. At the same time, overexpression of the insulin...
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Antibody H3K4me3 (C15410003)

5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming.
Published: March, 2011

Abstract: The epigenomes of early mammalian embryos are extensively reprogrammed to acquire a totipotent developmental potential. A major initial event in this reprogramming is the active loss/demethylation of 5-methylcytosine (5mC) in the zygote. Here, we ...
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Antibody

Epigenetic silencing mediated through activated PI3K/AKT signaling in breast cancer.
Published: March, 2011

Abstract: Trimethylation of histone 3 lysine 27 (H3K27me3) is a critical epigenetic mark for the maintenance of gene silencing. Additional accumulation of DNA methylation in target loci is thought to cooperatively support this epigenetic silencing during tu...
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Antibody 5mC (C15200081)

Embryonic lethal phenotype reveals a function of TDG in maintaining epigenetic stability.
Published: February, 2011

Abstract: Thymine DNA glycosylase (TDG) is a member of the uracil DNA glycosylase (UDG) superfamily of DNA repair enzymes. Owing to its ability to excise thymine when mispaired with guanine, it was proposed to act against the mutability of 5-methylcytosine ...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody

Characterization of the contradictory chromatin signatures at the 3' exons of zinc finger genes.
Published: February, 2011

Abstract: The H3K9me3 histone modification is often found at promoter regions, where it functions to repress transcription. However, we have previously shown that 3' exons of zinc finger genes (ZNFs) are marked by high levels of H3K9me3. We have now further...
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Bioruptor Chromatin Shearing ChIP-seq Antibody

Epigenetic control of vascular smooth muscle cells in Marfan and non-Marfan thoracic aortic aneurysms.
Published: February, 2011

Abstract: AIMS: Human thoracic aortic aneurysms (TAAs) are characterized by extracellular matrix breakdown associated with progressive smooth muscle cell (SMC) rarefaction. These features are present in all types of TAA: monogenic forms [mainly Marfan syndr...
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Antibody Histone ChIP kit

Comprehensive analysis of DNA-methylation in mammalian tissues using MeDIP-chip.
Published: February, 2011

Abstract: Genome-wide mapping of epigenetic changes is essential for understanding the mechanisms involved in gene regulation during cell differentiation and embryonic development. DNA-methylation is one of these key epigenetic marks that is directly linked...
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DNA shearing Bioruptor Antibody

Recombineering BAC transgenes for protein tagging.
Published: February, 2011

Abstract: Protein tagging offers many advantages for proteomic and regulomic research, particularly due to the use of generic and highly sensitive methods that can be applied with reasonable throughput. Ideally, protein tagging is equivalent to having a hig...
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Bioruptor Cell Lysis Western Blot

The histone methyltransferase Dot1 is required for DNA damage repair and proper development in Dictyostelium.
Published: January, 2011

Abstract: Posttranslational histone modifications play an important role in modulating gene expression and chromatin structure. Here we report the identification of histone H3K79 dimethylation in the simple eukaryote Dictyostelium discoideum. We have delete...
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Antibody

Inhibition of suppressive T cell factor 1 (TCF-1) isoforms in naive CD4+ T cells is mediated by IL-4/STAT6 signaling.
Published: January, 2011

Abstract: The Wnt pathway transcription factor T cell factor 1 (TCF-1) plays essential roles in the control of several developmental processes, including T cell development in the thymus. Although previously regarded as being required only during early T ce...
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Antibody LowCell ChIP kit

Characterisation of genome-wide PLZF/RARA target genes.
Published: January, 2011

Abstract: The PLZF/RARA fusion protein generated by the t(11;17)(q23;q21) translocation in acute promyelocytic leukaemia (APL) is believed to act as an oncogenic transcriptional regulator recruiting epigenetic factors to genes important for its transforming...
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Bioruptor Chromatin Shearing Antibody H3K4me3 (C15410003)

Role of p53 serine 46 in p53 target gene regulation.
Published: January, 2011

Abstract: The tumor suppressor p53 plays a crucial role in cellular growth control inducing a plethora of different response pathways. The molecular mechanisms that discriminate between the distinct p53-responses have remained largely elusive. Here, we have...
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Bioruptor Chromatin Shearing ChIP-seq Antibody

Enhanced chromatin accessibility and recruitment of JUNB mediate the sustained IL-4 expression in NFAT1 deficient T helper 2 cells.
Published: January, 2011

Abstract: Nuclear factor of activated T cells (NFAT) is a family of transcription factors composed of five proteins. Among them, NFAT1 is a predominant NFAT protein in CD4(+) T cells. NFAT1 positively regulates transcription of a large number of inducible c...
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DNA shearing Bioruptor Antibody

Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays.
Published: January, 2011

Abstract: UNLABELLED: ABSTRACT: BACKGROUND: The processes that compose expression of a given gene are far more complex than previously thought presenting unprecedented conceptual and mechanistic challenges that require development of new tools. Chromatin st...
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Bioruptor Chromatin Shearing Antibody

Epigenetic regulation of miR-212 expression in lung cancer.
Published: January, 2011

Abstract: Many studies have shown that microRNA expression in cancer may be regulated by epigenetic events. Recently, we found that in lung cancer miR-212 was strongly down-regulated. However, mechanisms involved in the regulation of miR-212 expression are ...
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Chromatin Shearing ChIP-qPCR Antibody LowCell ChIP kit

Epigenetic activation of SOX11 in lymphoid neoplasms by histone modifications.
Published: January, 2011

Abstract: Recent studies have shown aberrant expression of SOX11 in various types of aggressive B-cell neoplasms. To elucidate the molecular mechanisms leading to such deregulation, we performed a comprehensive SOX11 gene expression and epigenetic study in ...
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Antibody LowCell ChIP kit

Proteomic Analysis of Anti-inflammatory Effects of a Kampo (Japanese Herbal) Medicine "Shoseiryuto (Xiao-Qing-Long-Tang)" on Airway Inflammation in a Mouse Model.
Published: January, 2011

Abstract: Effects of a Kampo (Japanese herbal) medicine "shoseiryuto (SST, xiao-qing-long-tang in Chinese)", which has been used for the treatment of allergic bronchial asthma clinically, were examined on ovalbumin (OVA)-sensitized allergic airway inflammat...
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Tissue Homogenization

Epigenetic regulation on the 5'-proximal CpG island of human porcine endogenous retrovirus subgroup A receptor 2/GPR172B.
Published: January, 2011

Abstract: Porcine endogenous retroviruses (PERVs) have been considered one of the major risks of xenotransplantation from pigs to humans. PERV-A efficiently utilizes human PERV-A receptor 2 (HuPAR-2)/GPR172B to infect human cells; however, there has been no...
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Antibody

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