Publications

Explore Diagenode’s publication database. Learn how our products are used in diverse fields of research.

1425 publications found×

Analysis of active chromatin modifications in early mammalian embryos reveals uncoupling of H2A.Z acetylation and H3K36 trimethylation from embryonic genome activation

Abstract: Early embryonic development is characterized by dramatic changes in cell potency and chromatin organization. The role of histone variants in the context of chromatin remodeling during embryogenesis remains under investigated. In particular, the nu...
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Antibody H3K4me3 (C15410003)

Regulation of EP receptors in non-small cell lung cancer by epigenetic modifications.

Abstract: BACKGROUND: Cyclooxygenase (COX)-2 is frequently overexpressed in non-small cell lung cancer (NSCLC) and results in increased levels of prostaglandin E2 (PGE(2)), an important signalling molecule implicated in tumourigenesis. PGE(2) exerts its ef...
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Antibody

Whole genome methylation profiling by immunoprecipitation of methylated DNA

Abstract: I provide a protocol for DNA methylation profiling based on immunoprecipitation of methylated DNA using commercially available monoclonal antibodies that specifically recognize 5-methylcytosine. Quantification of the level of enrichment of the res...
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Antibody

Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells.

Abstract: Methylated DNA immunoprecipitation (MeDIP) is an immunocapturing approach for unbiased enrichment of DNA that is methylated on cytosines. The principle is that genomic DNA is randomly sheared by sonication and immunoprecipitated with an antibody t...
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DNA shearing Bioruptor Antibody 5mC (C15200081)

Role of Transcriptional Corepressor CtBP1 in Prostate Cancer Progression

Abstract: Transcriptional repressors and corepressors play a critical role in cellular homeostasis and are frequently altered in cancer. C-terminal binding protein 1 (CtBP1), a transcriptional corepressor that regulates the expression of tumor suppressors a...
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Antibody

Chromatin immunoprecipitation analysis in filamentous fungi.

Abstract: Chromatin immunoprecipitation (ChIP) is used to map the interaction between proteins and DNA at a specific genomic locus in the living cell. The protein-DNA complexes are stabilized already in vivo by reversible crosslinking and the DNA is sheared...
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Bioruptor Chromatin Shearing ChIP-qPCR Antibody

A novel mechanism for CTCF in the epigenetic regulation of Bax in breast cancer cells.

Abstract: We previously reported the association of elevated levels of the multifunctional transcription factor, CCCTC binding factor (CTCF), in breast cancer cells with the specific anti-apoptotic function of CTCF. To understand the molecular mechanisms of...
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Antibody H3K9ac (C15200185)

Experimental Study on Malignant Transformation of Human Bronchial Epithelial Cells Induced by Glycidyl Methacrylate and Analysis on its Methylation

Abstract: Objective To establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA) and define the different methylation genes at different stages. Methods DNA was extracted at different 16...
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DNA shearing Bioruptor Antibody

Detection of DNA methylation by MeDIP and MBDCap assays: an overview of techniques.

Abstract: DNA methylation has been characterized as the representative example of epigenetic modifications and implicated in numerous biological processes, such as genomic imprinting and X chromosome inactivation. It primarily occurs at CpG dinucleotides in...
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DNA shearing Bioruptor Antibody

Epigenetic-Mediated Downregulation of μ-Protocadherin in Colorectal Tumours

Abstract: Carcinogenesis involves altered cellular interaction and tissue morphology that partly arise from aberrant expression of cadherins. Mucin-like protocadherin is implicated in intercellular adhesion and its expression was found decreased in colorect...
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Bioruptor Chromatin Shearing Antibody H3K4me3 (C15410003) Bioruptor Plus

Esculetin Downregulates the Expression of AML1-ETO and C-Kit in Kasumi-1 Cell Line by Decreasing Half-Life of mRNA

Abstract: One of the most frequent genetic aberrations in acute myeloid leukemia (AML) is chromosomal translocation between AML1/RUNX1 on chromosome 21 and ETO gene on chromosome 8 resulting in the expression of chimeric oncogene AML1-ETO. Although patients...
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Antibody

A self-inactivating system for AAV-mediated in vivo base editing

Abstract: DNA base editors have been harnessed as an exciting therapeutic platform for human diseases and are rapidly progressing into human clinical trials. However, persistent expression of base editors delivered via adeno-associated virus (AAV) poses con...
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Antibody CRISPR

BET protein inhibition sensitizes glioblastoma cells to temozolomidetreatment by attenuating MGMT expression

Abstract: Bromodomain and extra-terminal tail (BET) proteins have been identified as potential epigenetic targets in cancer, including glioblastoma. These epigenetic modifiers link the histone code to gene transcription that can be disrupted with small mole...
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Bioruptor Antibody iDeal ChIP-seq Kit for Transcription Factors

PRAME induces genomic instability in uveal melanoma

Abstract: PRAME is a CUL2 ubiquitin ligase subunit that is normally expressed in the testis but becomes aberrantly overexpressed in many cancer types in association with aneuploidy and metastasis. Here, we show that PRAME is expressed predominantly in sperm...
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Antibody

Nucleophosmin 1 cooperates with the methyltransferase DOT1L toregulate H3K79me2 levels and DNA satellites expression atperi-nucleolar heterochromatin

Abstract: The histone methyltransferase DOT1L catalyzes methylation of H3K79 and it is highly conserved in mammals. DOT1L plays a functional role in several biological processes including cell cycle regulation, DNA repair, RNA splicing and gene expression, ...
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Bioruptor Antibody

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