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Comprehensive analysis of DNA-methylation in mammalian tissues using MeDIP-chip.


Pälmke N, Santacruz D, Walter J

Genome-wide mapping of epigenetic changes is essential for understanding the mechanisms involved in gene regulation during cell differentiation and embryonic development. DNA-methylation is one of these key epigenetic marks that is directly linked to gene expression is. Methylated DNA immunoprecipitation (MeDIP) is a recently devised method used to determine the distribution of DNA-methylation within functional regions (e.g., promoters) or in the entire genome robustly and cost-efficiently. This approach is based on the enrichment of methylated DNA with an antibody that specifically binds to 5-methyl-cytosine and can be combined with PCR, microarrays or high-throughput sequencing. This article outlines the experimental procedure of MeDIP-chip and provides a comprehensive summary of quality control strategies and primary data analysis.

Tags
DNA shearing
Bioruptor
Antibody

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Published
February, 2011

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Products used in this publication

  • RFX5-polyclonal-antibody-diagenode
    C15200006-100
    5-methylcytosine (5-mC) Antibody - cl. b

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