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Combining the use of the iDeal® CUT&Tag kit and the Tissue Nuclei Isolation Module for chromatin profiling in iPSC-derived cortical brain organoids.
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Application note: Best Workflow Practices for ChIP-seq Analysis with Small Samples
Combined chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) has become the gold standard to investigate genome-wide epigenetic profiles. However, ChIP from a limited amou...
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Chromatin shearing with the Diagenode One
The Diagenode One is a new affordable solution for Chromatin shearing in ChIP analysis for small samples. Designed to fit any bench, it is the smallest and lightest Diagenode shearing de...
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Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing
Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation of most archived pathological specimens. Archival FFPE samples provide an invaluable repository of in...
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LC-MS measurement of whole-body uptake of pharmaceuticals in zebrafish using the Bioruptor® Plus for sample preparation
The laboratory for Pharmaceutical Analysis at KU Leuven is developing innovative separation and detection techniques in pharmaceutical applications. Within the Department of Pharmaceutical and Phar...
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Optimal long DNA fragment generation prior to SMRTbell® library preparation
Several methods exist today for shearing genomic DNA, but few are reliable for generating DNA fragments >50 kb necessary for assembly of many microbial, human, plant and animal genomes de novo. ...
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Efficient and streamlined solution for high-quality NGS library preparation using the Bioruptor® Pico and JetSeqTM Flex DNA Library Preparation Kit
Next Generation Sequencing workflows include a number of critical steps which can have a significant impact both on the quality of the results and on the overall cost of the project. In particular,...
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Comparison of the S. cerevisae chromatin shearing with Covaris™ S2 and Bioruptor® Pico
The chromatin shearing is very important step for all kind of ChIP analysis followed by next-generation sequencing. The efficiency, reproducibility and straightforwardness are the most important ch...
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Ultrasensitive proteomics for microdissected fresh frozen tissue samples
This protocol, specifically designed for ultrasensitive proteomics, enables high throughput quantitative proteomics study on microdissected tissue samples. The Bioruptor® Pico provided high eff...
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Optimize the selection of guide RNA by ChIP to keep CRISPR on-target
The mechanisms of target recognition and target specificity of the Cas9 protein is still not completely understood. A major hurdle of this technology is the introduction of double-strand breaks (DS...
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Sequence of the methylated spike-in control in FASTA format
This document and files are to process the sequencing data from the spike-in controls included in the RRBS kit in order to estimate the bisulfite conversion efficiency. You can find the sequences o...
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Sequence of the unmethylated spike-in control in FASTA format
This document and files are to process the sequencing data from the spike-in controls included in the RRBS kit in order to estimate the bisulfite conversion efficiency. You can find the sequences o...
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Positions of the unmethylated cytosines in the sequence of the methylated control in BED format
This document and files are to process the sequencing data from the spike-in controls included in the RRBS kit in order to estimate the bisulfite conversion efficiency. You can find the sequences o...
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acrIIA2 monoclonal antibody datasheet
Monoclonal antibody raised in mouse against acrIIA2 (anti CRISPR protein IIA2) using the full length recombinant protein.
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ChIP workshop 08/12/2020
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ChIP workshop 16-17/02/2021
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Compare Bioruptor Features (Tables)
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Diagenode Bioruptor DNA QC kit analysis template
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ChIP workshop 15/06/2021
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RARA antibody
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