The iDeal CUT&Tag kit has been designed for efficient chromatin profiling of histone marks and some non-histone proteins. CUT&Tag is a recently developed strategy to profile the location of chromatin components genome-wide (1). The method selectively targets chromatin-bound proteins with specific antibodies in intact nuclei or cells, without fragmentation or immunoprecipitation. It offers many advantages, including a low background and the requirement of low cell inputs and sequencing depth. The team of Gabriel Santpere at Hospital del Mar Research Institute in Barcelona is focused on the experimental determination and analysis of transcription factor (TF) binding sites during neurogenesis and neuronal differentiation (2). In this application note, we detail the procedure and present the results we have obtained from validating the combination of the iDeal CUT&Tag kit and the Tissue Nuclei Extraction Module on cortical organoids derived from induced pluripotent stem cells (iPSC) to test specific TFs. We show that the iDeal CUT&Tag kit for histones together with the Tissue Nuclei Extraction Module can be used to ascertain the genome-wide binding landscape of both histone associated proteins and transcription factors in iPSC-derived cortical brain organoids. Read more.
Additionally, you may find this Application Note helpful: Mapping Histone post-translational modifications in the Pacific oyster using iDeal CUT&Tag and the Tissue Nuclei Extraction Module.
