Documents
Download Automated method for RNAseq libraries
An automated method for efficient, accurate and reproducible construction of RNA-seq libraries
Download ChIP-seq from human tumor tissue
Application of the Bioruptor® Pico for shearing chromatin fragments for ChIP-seq from human tumor tissue.
Over the last several years, chromatin immunoprecipitation followed by massive paralle...
Download Automated ChIP with IPStar
Screening histone modifications in genomic regions using chromatin from 1,000 to 1 million cells
Download Spatial tissue proteomics of FFPE specimen using the Bioruptor® for sample preparation
Thanks to the excellent preservation of morphological features, formalin fixed and paraffin embedded (FFPE) tissues are an invaluable resource for spatial tissue proteomics. At the same time, due t...
Download Application of the Megaruptor® for shearing of ultra-long DNA fragments in the context of Long Read Sequencing using 3rd Gen Sequencing Technologies
The Megaruptor® provides a simple, robust, and straight forward method for reproducible DNA shearing to obtain ultra-large DNA fragments. The system has been easily integrated into our workflow...
Download Successful application of novel plant ChIP-seq technology results in high quality sequencing data from Arabidopsis thaliana seedling cells
In the recent years massively parallel sequencing (also known as Next Generation Sequencing or NGS) has become the dominant method for sequencing, and the decreasing costs and increasing yields tra...
Download Bacterial Cell Disruption using Bioruptor® Plus
Disruption of bacterial cells is required for releasing biological molecules from within the cells for various biological applications. Ultrasound technology has been widely used for such c...
Download Evaluation of Bioruptor (Diagenode) in Shotgun Library Preparation for Massively Parallel Sequencing on Illumina Genome Analyzer II
Contributed by Choli Lee - Shendure Lab - Department of Genome Sciences - University of Washington - Seattle, WA. The Illumina Genome Analyzer II (GA-II) performs massiv...
Download Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology
Dr. Kelvin Luk’s lab at the University of Pennsylvania focuses on understanding Parkinson’s disease (PD), a progressive neurodegenerative condition for which there is currently no cure....
Download Megaruptor genomic DNA shearing for large insert sequencing library preparation
Wageningen University has a strong background in plant genome sequencing including contributions to the Arabidopsis, potato and tomato genome research as partner of various international consortia....
Download Diagenode Premium RRBS technology: Cost-effective DNA methylation mapping with superior CpG resolution and coverage
DNA methylation is an important epigenetic mark with broad relevance in development and disease. Reduced representation bisulfite sequencing (RRBS) enables genome-scale DNA methylation analysis wit...
Download Application note: D-Plex Small RNA DNBSEQ™ Kit for MGI
Download Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit
By combining the optimized chemistry of the Arima-HiC kit along with new low input protocols, the potential applications of powerful Hi-C technology are unlocked. When studying samples that are dif...
Download Application note - ChIP on yeast
The Application Note: "Use of the iDeal® chromatin immunoprecipitation (ChIP) kit for the identification of transcription factor binding sites in the pathogenic yeast Candida glabrata" presents...
Download Using the DNAFluid+ Kit for viscous DNA to effectively pre-condition high molecular weight DNA for consistent shearing
This application note demonstrates that the PacBio Circulomics SRE XS is a good substitute for gel-based size selection and that the DNAFluid+ works well to pre-condition viscous DNA for Long Hydro...
Download Application of the Bioruptor® Pico for best shearing of yeast genomic DNA for NGS
DNA shearing is important step for next-generation sequencing library preparation. Important is reproducibility and ef ciency of shearing. S. cerevisiae is yeast and have smaller amount of DNA per ...
Download Sage Science size selection with Diagenode Megaruptor
Best practices: Sage Science size selection with Diagenode Megaruptor shearing for long-read sequencing library preparation
Download Combining the use of the iDeal® CUT&Tag kit and the Tissue Nuclei Isolation Module for chromatin profiling in iPSC-derived cortical brain organoids.
Download Application note: Best Workflow Practices for ChIP-seq Analysis with Small Samples
Combined chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) has become the gold standard to investigate genome-wide epigenetic profiles. However, ChIP from a limited amou...
Download Chromatin shearing with the Diagenode One
The Diagenode One is a new affordable solution for Chromatin shearing in ChIP analysis for small samples. Designed to fit any bench, it is the smallest and lightest Diagenode shearing de...