Application of the Megaruptor® for shearing of ultra-long DNA fragments in the context of Long Read Sequencing using 3rd Gen Sequencing Technologies
The Megaruptor® provides a simple, robust, and straight forward method for reproducible DNA shearing to obtain ultra-large DNA fragments. The system has been easily integrated into our workflow for long range sequencing of single molecules using the RSII platform from Pacific Biosciences®. The obtained dense distribution of fragment sizes after shearing and the low content of non-sheared as well as short fragments was beneficial for the performance of the whole library preparation process.
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