Application of the Bioruptor®Pico for shearing chromatin fragments for ChIP-seq from human tumor tissue.
Over the last several years, chromatin immunoprecipitation followed by massive parallel sequencing (ChIP-seq) has helped to identify transcription factor function and epigenetic modifications on a genome-wide scale. Most of these studies have classically been performed using cell line material. In this application note, we illustrate that the Bioruptor®Pico (Diagenode) can process various human tumor tissue for ChIP-seq analyses of transcription factors as well as epigenetic marks.
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