Diagenode

Ty1 monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200054
(MAb-054-050)
50 µg/29 µl
$295.00
  Bulk order

Monoclonal antibody raised in mouse against the Ty1 tag (amino acid sequence EVHTNQDPLD).

Lot005
Concentration1.73 µg/µl
Species reactivity
TypeMonoclonal
PurityProtein A purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2 μg/ChIP Fig 1
Western Blotting 1:1,000 - 1:2,000 Fig 2
* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
  • Validation Data
    ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody against the Ty1 tag
    ChIP assays were performed using Plasmodium falciparium cells stably expressing Ty1-H2A (blue bars) and wild type Plasmodium falciparium cells (brown bars). Primer pairs were used for active genes, inactive genes, and variable genes. Sheared chromatin from 1x10e6 cells and 2 μg of antibody against Ty1 (cat. No. C15200054) were used per ChIP experiment

    Western blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody against the Ty1 tag
    Western blot was performed on 25 μg whole cell extracts from HeLa cells using the antibody against the Ty1 tag (cat. No. C15200054) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Figure 2 shows the results for untransfected cells, used as a negative control (lane 1 and 4) and for cells transfected with a Ty1-tagged MBD1 (lane 2) and a Ty1-tagged MBD2 (lane 3). The marker (in kDa) is shown on the left.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet Ty1 C15200054 DATASHEET
    Monoclonal antibody raised in mouse against the Ty1 tag (amino acid sequence EVHTNQDPLD).
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Ty1 monoclonal antibody - Classic (Diagenode Cat# C15200054 Lot# 005). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Severe muscle wasting and denervation in mice lacking the RNA-binding protein ZFP106
    Anderson DM et al.
    Innervation of skeletal muscle by motor neurons occurs through the neuromuscular junction, a cholinergic synapse essential for normal muscle growth and function. Defects in nerve-muscle signaling cause a variety of neuromuscular disorders with features of ataxia, paralysis, skeletal muscle wasting, and degeneration....

    Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals
    Wu H et al.
    For genetically heterogeneous diseases a better understanding of how the underlying gene defects are functionally interconnected will be important for dissecting disease etiology. The Immunodeficiency, Centromeric instability, Facial anomalies (ICF) syndrome is a chromatin disorder characterized by mutations in DNMT...

    Osr1 Interacts Synergistically with Wt1 to Regulate Kidney Organogenesis
    Xu J et al.
    Renal hypoplasia is a common cause of pediatric renal failure and several adult-onset diseases. Recent studies have associated a variant of the OSR1 gene with reduction of newborn kidney size and function in heterozygotes and neonatal lethality with kidney defects in homozygotes. How OSR1 regulates kidney developmen...

    Zbtb20 promotes astrocytogenesis during neocortical development
    Nagao M, Ogata T, Sawada Y, Gotoh Y
    Multipotent neural precursor cells (NPCs) generate astrocytes at late stages of mammalian neocortical development. Many signalling pathways that regulate astrocytogenesis directly induce the expression of GFAP, a marker of terminally differentiated astrocytes. However, astrocyte specification occurs before GFAP expr...

    Expression of the MOZ-TIF2 oncoprotein in mice represses senescence.
    Largeot A, Perez-Campo FM, Marinopoulou E, Lie-A-Ling M, Kouskoff V, Lacaud G.
    The MOZ-TIF2 translocation, that fuses MOZ (Monocytic Leukemia Zinc finger protein) histone acetyltransferase (HAT) with the nuclear co-activator TIF2, is associated the development of Acute Myeloid Leukemia. We recently showed that in the absence of MOZ HAT activity, p16INK4atranscriptional levels are sig...

    The Cytoplasmic Region of Plasmodium falciparum SURFIN4.2 Is Required for Transport from Maurer’s Clefts to the Red Blood Cell Surface
    Kagaya W et al.
    Background: Plasmodium, the causative agent of malaria, exports many proteins to the surface of the infected red blood cell (iRBC) in order to modify it toward a structure more suitable for parasite development and survival. One such exported protein, SURFIN4.2, from the parasite of human malignant malaria, P. fal...

    TAL effector-mediated genome visualization (TGV).
    Miyanari Y
    The three-dimensional remodeling of chromatin within nucleus is being recognized as determinant for genome regulation. Recent technological advances in live imaging of chromosome loci begun to explore the biological roles of the movement of the chromatin within the nucleus. To facilitate better understanding of the ...

    Live visualization of chromatin dynamics with fluorescent TALEs.
    Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
    The spatiotemporal organization of genomes in the nucleus is an emerging key player to regulate genome function. Live imaging of nuclear organization dynamics would be a breakthrough toward uncovering the functional relevance and mechanisms regulating genome architecture. Here, we used transcription activator-like e...

    Utx is required for proper induction of ectoderm and mesoderm during differentiation of embryonic stem cells.
    Morales Torres C, Laugesen A, Helin K
    Embryonic development requires chromatin remodeling for dynamic regulation of gene expression patterns to ensure silencing of pluripotent transcription factors and activation of developmental regulators. Demethylation of H3K27me3 by the histone demethylases Utx and Jmjd3 is important for the activation of lineage ch...

    The N-terminal segment of Plasmodium falciparum SURFIN(4.1) is required for its trafficking to the red blood cell cytosol through the endoplasmic reticulum.
    Zhu X, Yahata K, Alexandre JS, Tsuboi T, Kaneko O
    Plasmodium falciparum SURFIN is a type I transmembrane protein that shares domains with molecules expressed on the surface of the red blood cells (RBCs) infected with a variety of malaria parasite species, such as P. falciparum PfEMP1, P. vivax VIR proteins, and P. knowlesi SICAvar. Thus, understanding the export me...

    The Human EKC/KEOPS Complex Is Recruited to Cullin2 Ubiquitin Ligases by the Human Tumour Antigen PRAME.
    Costessi A, Mahrour N, Sharma V, Stunnenberg R, Stoel MA, Tijchon E, Conaway JW, Conaway RC, Stunnenberg HG
    The human tumour antigen PRAME (preferentially expressed antigen in melanoma) is frequently overexpressed during oncogenesis, and high PRAME levels are associated with poor clinical outcome in a variety of cancers. However, the molecular pathways in which PRAME is implicated are not well understood. We recently char...

    An essential novel component of the non-canonical mitochondrial outer membrane protein import system of trypanosomatids.
    Pusnik M, Mani J, Schmidt O, Niemann M, Oeljeklaus S, Schnarwiler F, Warscheid B, Lithgow T, Meisinger C, Schneider A.
    The mitochondrial outer membrane protein Tom40 is the general entry gate for imported proteins in essentially all eukaryotes. Trypanosomatids however lack Tom40 and use instead a protein termed the archaic translocase of the outer mitochondrial membrane (ATOM). Here we report the discovery of pATOM36, a novel essent...

    Selective autophagy regulates insertional mutagenesis by the Ty1 retrotransposon in Saccharomyces cerevisiae.
    Suzuki K, Morimoto M, Kondo C, Ohsumi Y
    Macroautophagy (autophagy) is a bulk degradation system for cytoplasmic components and is ubiquitously found in eukaryotic cells. Autophagy is induced under starvation conditions and plays a cytoprotective role by degrading unwanted cytoplasmic materials. The Ty1 transposon, a member of the Ty1/copia superfamily, is...

    The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters.
    Costessi A, Mahrour N, Tijchon E, Stunnenberg R, Stoel MA, Jansen PW, Sela D, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC, Stunnenberg HG
    The human tumour antigen PRAME (preferentially expressed antigen of melanoma) is frequently overexpressed in tumours. High PRAME levels correlate with poor clinical outcome of several cancers, but the mechanisms by which PRAME could be involved in tumourigenesis remain largely elusive. We applied protein-complex pur...

    Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis.
    Hofemeister H, Ciotta G, Fu J, Seibert PM, Schulz A, Maresca M, Sarov M, Anastassiadis K, Stewart AF
    Protein tagging offers many advantages for proteomic and regulomic research. Ideally, protein tagging is equivalent to having a high affinity antibody for every chosen protein. However, these advantages are compromised if the tagged protein is overexpressed, which is usually the case from cDNA expression vectors. Ph...

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